Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1956008..1956510 Replicon chromosome
Accession NZ_CP072686
Organism Escherichia coli strain FP4

Toxin (Protein)


Gene name yoeB Uniprot ID D8A584
Locus tag J8I94_RS09410 Protein ID WP_000767828.1
Coordinates 1956256..1956510 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag J8I94_RS09405 Protein ID WP_001259255.1
Coordinates 1956008..1956259 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8I94_RS09380 (1951186) 1951186..1952253 - 1068 WP_000080129.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
J8I94_RS09385 (1952253) 1952253..1953323 - 1071 WP_000108949.1 histidinol-phosphate transaminase -
J8I94_RS09390 (1953320) 1953320..1954624 - 1305 WP_001370829.1 histidinol dehydrogenase -
J8I94_RS09395 (1954630) 1954630..1955529 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
J8I94_RS09400 (1955675) 1955675..1955725 - 51 WP_001364200.1 his operon leader peptide -
J8I94_RS09405 (1956008) 1956008..1956259 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
J8I94_RS09410 (1956256) 1956256..1956510 + 255 WP_000767828.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
J8I94_RS09415 (1956593) 1956593..1957417 + 825 WP_282478837.1 SDR family oxidoreductase -
J8I94_RS09420 (1957463) 1957463..1958392 + 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
J8I94_RS09425 (1958607) 1958607..1958669 + 63 WP_010723108.1 membrane protein YoeI -
J8I94_RS09430 (1958659) 1958659..1960017 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
J8I94_RS09435 (1960196) 1960196..1961254 + 1059 WP_000492324.1 thiosulfate utilization transporter TsuA/YeeE -
J8I94_RS09440 (1961268) 1961268..1961495 + 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10169.56 Da        Isoelectric Point: 8.0353

>T198238 WP_000767828.1 NZ_CP072686:1956256-1956510 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEDHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T198238 NZ_CP096843:c2833458-2833063 [Mycobacterium tuberculosis variant bovis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT198238 WP_001259255.1 NZ_CP072686:1956008-1956259 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT198238 NZ_CP096843:c2833700-2833455 [Mycobacterium tuberculosis variant bovis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G465

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References