Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3437464..3438082 Replicon chromosome
Accession NZ_CP072566
Organism Escherichia coli strain WG5::TT12A

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag J8F85_RS16600 Protein ID WP_001291435.1
Coordinates 3437864..3438082 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag J8F85_RS16595 Protein ID WP_000344800.1
Coordinates 3437464..3437838 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8F85_RS16585 (3432553) 3432553..3433746 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
J8F85_RS16590 (3433769) 3433769..3436918 + 3150 WP_081508855.1 efflux RND transporter permease AcrB -
J8F85_RS16595 (3437464) 3437464..3437838 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
J8F85_RS16600 (3437864) 3437864..3438082 + 219 WP_001291435.1 HHA domain-containing protein Toxin
J8F85_RS16605 (3438254) 3438254..3438805 + 552 WP_000102564.1 maltose O-acetyltransferase -
J8F85_RS16610 (3438921) 3438921..3439391 + 471 WP_000136192.1 YlaC family protein -
J8F85_RS16615 (3439555) 3439555..3441105 + 1551 WP_001408474.1 cyclic-guanylate-specific phosphodiesterase PdeB -
J8F85_RS16620 (3441147) 3441147..3441500 - 354 WP_000878140.1 DUF1428 domain-containing protein -
J8F85_RS16630 (3441879) 3441879..3442190 + 312 WP_000409911.1 MGMT family protein -
J8F85_RS16635 (3442221) 3442221..3442793 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T198112 WP_001291435.1 NZ_CP072566:3437864-3438082 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T198112 NZ_CP096841:2190732-2191109 [Mycobacterium tuberculosis variant bovis]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT198112 WP_000344800.1 NZ_CP072566:3437464-3437838 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT198112 NZ_CP096841:2191151-2191600 [Mycobacterium tuberculosis variant bovis]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References