Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2488610..2489248 Replicon chromosome
Accession NZ_CP072565
Organism Escherichia coli strain WG5::PA8

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag J8F86_RS12020 Protein ID WP_000813794.1
Coordinates 2489072..2489248 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag J8F86_RS12015 Protein ID WP_001270286.1
Coordinates 2488610..2489026 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8F86_RS11995 (2483762) 2483762..2484703 - 942 WP_001251304.1 ABC transporter permease -
J8F86_RS12000 (2484704) 2484704..2485717 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
J8F86_RS12005 (2485735) 2485735..2486880 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
J8F86_RS12010 (2487125) 2487125..2488531 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
J8F86_RS12015 (2488610) 2488610..2489026 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
J8F86_RS12020 (2489072) 2489072..2489248 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
J8F86_RS12025 (2489470) 2489470..2489700 + 231 WP_000494244.1 YncJ family protein -
J8F86_RS12030 (2489792) 2489792..2491753 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
J8F86_RS12035 (2491826) 2491826..2492362 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
J8F86_RS12040 (2492454) 2492454..2493629 + 1176 WP_001236265.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T198088 WP_000813794.1 NZ_CP072565:c2489248-2489072 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T198088 NZ_CP096841:c697570-697178 [Mycobacterium tuberculosis variant bovis]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT198088 WP_001270286.1 NZ_CP072565:c2489026-2488610 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT198088 NZ_CP096841:c697824-697567 [Mycobacterium tuberculosis variant bovis]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References