Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/ParE-RelB |
| Location | 2332517..2333043 | Replicon | chromosome |
| Accession | NZ_CP072564 | ||
| Organism | Escherichia coli strain WG5::PA2 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | - |
| Locus tag | J8F87_RS11280 | Protein ID | WP_047402633.1 |
| Coordinates | 2332756..2333043 (+) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | S1F6D3 |
| Locus tag | J8F87_RS11275 | Protein ID | WP_000534858.1 |
| Coordinates | 2332517..2332756 (+) | Length | 80 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| J8F87_RS11245 (2327621) | 2327621..2328042 | + | 422 | Protein_2207 | hypothetical protein | - |
| J8F87_RS11255 (2329923) | 2329923..2330945 | + | 1023 | Protein_2209 | ISNCY family transposase | - |
| J8F87_RS11260 (2331066) | 2331066..2331215 | + | 150 | WP_011443592.1 | protein YdfW | - |
| J8F87_RS11265 (2331652) | 2331652..2331984 | - | 333 | WP_001301033.1 | FlxA-like family protein | - |
| J8F87_RS11270 (2332187) | 2332187..2332492 | - | 306 | WP_001326990.1 | protein YdfV | - |
| J8F87_RS11275 (2332517) | 2332517..2332756 | + | 240 | WP_000534858.1 | type II toxin-antitoxin system antitoxin RelB | Antitoxin |
| J8F87_RS11280 (2332756) | 2332756..2333043 | + | 288 | WP_047402633.1 | type II toxin-antitoxin system mRNA interferase RelE | Toxin |
| J8F87_RS11285 (2333115) | 2333115..2333270 | + | 156 | WP_000813254.1 | type I toxin-antitoxin system toxin HokD | - |
| J8F87_RS11290 (2333487) | 2333487..2333738 | + | 252 | WP_000980994.1 | protein Rem | - |
| J8F87_RS11295 (2333805) | 2333805..2334083 | + | 279 | WP_012304870.1 | hypothetical protein | - |
| J8F87_RS11300 (2334085) | 2334085..2335134 | + | 1050 | WP_001265199.1 | DUF968 domain-containing protein | - |
| J8F87_RS11305 (2335148) | 2335148..2335900 | + | 753 | WP_001047135.1 | antitermination protein | - |
| J8F87_RS11310 (2336178) | 2336178..2336267 | - | 90 | WP_120795389.1 | hypothetical protein | - |
| J8F87_RS11315 (2336322) | 2336322..2336534 | - | 213 | WP_000087756.1 | cold shock-like protein CspF | - |
| J8F87_RS11320 (2336835) | 2336835..2337050 | + | 216 | WP_000066484.1 | cold shock-like protein CspB | - |
| J8F87_RS11325 (2337414) | 2337414..2337584 | - | 171 | WP_211180497.1 | putative zinc-binding protein YnfU | - |
| J8F87_RS11330 (2337804) | 2337804..2338019 | + | 216 | WP_000839590.1 | phage lysis protein EssD | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 2320429..2354540 | 34111 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11224.28 Da Isoelectric Point: 10.4221
>T198063 WP_047402633.1 NZ_CP072564:2332756-2333043 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLKSPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLKSPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL
Download Length: 288 bp
>T198063 NZ_CP096840:c2384205-2383888 [Mycobacterium tuberculosis variant bovis]
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA
Antitoxin
Download Length: 80 a.a. Molecular weight: 9071.48 Da Isoelectric Point: 4.5230
>AT198063 WP_000534858.1 NZ_CP072564:2332517-2332756 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL
Download Length: 240 bp
>AT198063 NZ_CP096840:c2384417-2384202 [Mycobacterium tuberculosis variant bovis]
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|---|---|
| PDB | 4FXE | |
| PDB | 2KC8 | |
| PDB | 2K29 | |
| AlphaFold DB | A0A4V1CTS8 |