Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4048288..4048907 Replicon chromosome
Accession NZ_CP072413
Organism Klebsiella pneumoniae strain B32278

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag J6256_RS19850 Protein ID WP_002892050.1
Coordinates 4048689..4048907 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag J6256_RS19845 Protein ID WP_002892066.1
Coordinates 4048288..4048662 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6256_RS19835 (4043440) 4043440..4044633 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
J6256_RS19840 (4044656) 4044656..4047802 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
J6256_RS19845 (4048288) 4048288..4048662 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
J6256_RS19850 (4048689) 4048689..4048907 + 219 WP_002892050.1 HHA domain-containing protein Toxin
J6256_RS19855 (4049070) 4049070..4049636 + 567 WP_002892030.1 maltose O-acetyltransferase -
J6256_RS19860 (4049608) 4049608..4049748 - 141 WP_004147370.1 hypothetical protein -
J6256_RS19865 (4049769) 4049769..4050239 + 471 WP_002892026.1 YlaC family protein -
J6256_RS19870 (4050214) 4050214..4051665 - 1452 WP_064162152.1 PLP-dependent aminotransferase family protein -
J6256_RS19875 (4051766) 4051766..4052464 + 699 WP_004177238.1 GNAT family protein -
J6256_RS19880 (4052461) 4052461..4052601 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
J6256_RS19885 (4052601) 4052601..4052864 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T197743 WP_002892050.1 NZ_CP072413:4048689-4048907 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T197743 NZ_CP096666:c1073923-1073720 [Yersinia pestis EV76-CN]
ATGACAAAAACTGACTACCTGATGCGTTTAAGAAAATGTACGACTATCGATACGCTAGAACGTGTAATTGAAAAAAATAA
GTACGAACTTTCTGATGATGAACTGGAATTGTTTTACTCAGCTGCAGACCATCGCCTGGCTGAACTAACGATGAATAAGC
TTTATGATAAAATTCCACCTACTGTATGGCAACATGTGAAATAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT197743 WP_002892066.1 NZ_CP072413:4048288-4048662 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT197743 NZ_CP096666:c1074340-1073972 [Yersinia pestis EV76-CN]
ATGGATGAGTACTCGCCTAAGCGGCATGACATTGCCCAACTGAAGTTTCTATGTGAAAGCTTGTATGATGAAGGTATTGC
AACTCTTGGCGACAGCCATCATGGCTGGATAAATGACCCAACGTCGGCCATAAACCTTCAACTCAATGATTTGATCGAGC
ATATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACCCGGATGGAAGCCAGCTTTCCGAGTTAGTGGAAGAATATTTA
GACGACACATATACTCTGTTTAGTAGTTATGGAATCAATGATCCTGAATTGCGTCGCTGGCAGAAAACCAAAGAGCATTT
ATTCAGACTTTTCTCAGGGGAATACGTATGTACCCTGATGAAAACTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References