Detailed information of TA system TA197725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2676962..2678550
• Replicon: chromosome
• Accession: NZ_CP072408
• Organism: Klebsiella pneumoniae strain B4650

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: J6316_RS13090
• Protein ID: WP_064162061.1
• Coordinates: 2676962..2678284 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: J6316_RS13095
• Protein ID: WP_002904790.1
• Coordinates: 2678284..2678550 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6316_RS13075 (2672018)	2672018..2674519	+	2502	WP_064162059.1	fimbria/pilus outer membrane usher protein	-
J6316_RS13080 (2674507)	2674507..2675511	+	1005	WP_064162060.1	fimbrial protein	-
J6316_RS13085 (2675653)	2675653..2676945	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
J6316_RS13090 (2676962)	2676962..2678284	-	1323	WP_064162061.1	type II toxin-antitoxin system HipA family toxin	Toxin
J6316_RS13095 (2678284)	2678284..2678550	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
J6316_RS13100 (2678816)	2678816..2679142	-	327	WP_064162062.1	hydroxyisourate hydrolase	-
J6316_RS13105 (2679139)	2679139..2679639	-	501	WP_064162063.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
J6316_RS13110 (2679617)	2679617..2680996	-	1380	WP_040245186.1	nucleobase:cation symporter-2 family protein	-
J6316_RS13115 (2681342)	2681342..2682496	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
J6316_RS13120 (2682477)	2682477..2683400	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49155.46 Da
• Isoelectric Point: 7.5357

>T197725 WP_064162061.1 NZ_CP072408:c2678284-2676962 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSTHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T197725 NZ_CP096594:742570-742845 [Paracoccus denitrificans]
GTGCCTGAGGTGGAATGGCGGCAAGCCGCGCGCGAGGACCTGCGTTCGATCTTCGAGTACATCGCCGATGACAATCCGAC
AGCGGCACTTGCGCTGCTGGATGAGATCGAGGCAAAGGTTGGCAAGTTGCCTGATCGGCTTAGAGCGTATCGGCCGGGCA
GGGTGACAGGGACACGCGAAATGGTCGTCCGGCCTAACTATCTTGTGGTCTATGCTGAGGTCGGGGGAGCGCTGGTGATC
CTGCGGGTCTTGCATGCGGCGCAGATGTGGCCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT197725 WP_002904790.1 NZ_CP072408:c2678550-2678284 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT197725 NZ_CP096594:742284-742577 [Paracoccus denitrificans]
ATGCCCGCACAAACTTCGATGCTGCACGTCCGCGTGGACGACCAACTCAAGGCCCAAGCTGCTGAGGCGCTGGCTGGCGT
CGGCCTGACCCTGTCGGATGCGGTTCGCATCCTGTTGACCCGCGTTGCGCGGGAGGGCGGGTTGCCGGCCGGTTTGACAG
CCGACCCGGACGTCTATGACGCGTGGTTCCGCGCCAAGGTTCGCGAAGCGCTTGAGGATGACCAGCCGACCATCCCGCAT
GTCGATATCATGGCGGAAATGCGCGAACTGATCGAGCGGAAGCGCCGTGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GXD8