Detailed information of TA system TA197624

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 39680..40106
• Replicon: plasmid pE105-6
• Accession: NZ_CP072317
• Organism: Escherichia coli strain E105

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: J0O73_RS26750
• Protein ID: WP_001372321.1
• Coordinates: 39680..39805 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	39882..40106 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0O73_RS26715 (34703)	34703..34930	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
J0O73_RS26720 (35067)	35067..35738	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
J0O73_RS26725 (35932)	35932..36315	-	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
J0O73_RS26730 (36638)	36638..37240	+	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
J0O73_RS26735 (37537)	37537..38358	-	822	WP_001234445.1	DUF932 domain-containing protein	-
J0O73_RS26740 (38469)	38469..38765	-	297	WP_001272251.1	hypothetical protein	-
J0O73_RS26745 (39065)	39065..39361	+	297	Protein_46	hypothetical protein	-
J0O73_RS26750 (39680)	39680..39805	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
J0O73_RS27445 (39747)	39747..39896	-	150	Protein_48	plasmid maintenance protein Mok	-
J0O73_RS26755 (39918)	39918..40097	+	180	WP_001309233.1	hypothetical protein	-
- (39882)	39882..40106	-	225	NuclAT_0	-	Antitoxin
- (39882)	39882..40106	-	225	NuclAT_0	-	Antitoxin
- (39882)	39882..40106	-	225	NuclAT_0	-	Antitoxin
- (39882)	39882..40106	-	225	NuclAT_0	-	Antitoxin
J0O73_RS26760 (40075)	40075..40837	-	763	Protein_50	plasmid SOS inhibition protein A	-
J0O73_RS26765 (40834)	40834..41268	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
J0O73_RS26770 (41337)	41337..43360	-	2024	Protein_52	ParB/RepB/Spo0J family partition protein	-
J0O73_RS26775 (43421)	43421..43654	-	234	WP_000006003.1	DUF905 family protein	-
J0O73_RS26780 (43712)	43712..44239	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
J0O73_RS26785 (44541)	44541..45008	+	468	Protein_55	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T197624 WP_001372321.1 NZ_CP072317:c39805-39680 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T197624 NZ_CP096274:2396558-2396824 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT197624 NZ_CP072317:c40106-39882 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin