Detailed information of TA system TA197567

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 63965..64623
• Replicon: plasmid p2KSK6
• Accession: NZ_CP072272
• Organism: Acinetobacter baumannii strain KSK6

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: J6E47_RS21040
• Protein ID: WP_000312250.1
• Coordinates: 64264..64623 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J6E47_RS21035
• Protein ID: WP_001096429.1
• Coordinates: 63965..64264 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6E47_RS20995 (J6E47_20995)	58990..60270	+	1281	WP_162200943.1	hypothetical protein	-
J6E47_RS21000 (J6E47_21000)	60375..60632	+	258	WP_000834290.1	hypothetical protein	-
J6E47_RS21005 (J6E47_21005)	60637..61209	+	573	WP_000443897.1	hypothetical protein	-
J6E47_RS21010 (J6E47_21010)	61185..61364	+	180	WP_000387630.1	hypothetical protein	-
J6E47_RS21015 (J6E47_21015)	61381..62010	+	630	WP_000701003.1	hypothetical protein	-
J6E47_RS21020 (J6E47_21020)	62050..62268	+	219	WP_001043201.1	hypothetical protein	-
J6E47_RS21025 (J6E47_21025)	62288..62842	+	555	WP_000790084.1	hypothetical protein	-
J6E47_RS21030 (J6E47_21030)	62892..63428	+	537	WP_000731978.1	hypothetical protein	-
J6E47_RS21035 (J6E47_21035)	63965..64264	-	300	WP_001096429.1	XRE family transcriptional regulator	Antitoxin
J6E47_RS21040 (J6E47_21040)	64264..64623	-	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J6E47_RS21045 (J6E47_21045)	64824..65390	+	567	WP_000710385.1	hypothetical protein	-
J6E47_RS21050 (J6E47_21050)	65439..65621	+	183	WP_000373385.1	hypothetical protein	-
J6E47_RS21055 (J6E47_21055)	65688..66386	+	699	WP_000873188.1	hypothetical protein	-
J6E47_RS21060 (J6E47_21060)	66525..66908	+	384	WP_000654348.1	hypothetical protein	-
J6E47_RS21065 (J6E47_21065)	66975..67733	+	759	WP_001053127.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-VIa	-	1..68224	68224

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T197567 WP_000312250.1 NZ_CP072272:c64623-64264 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T197567 NZ_CP096255:7594639-7594992 [Bradyrhizobium barranii subsp. apii]
ATGAAGCGAGGTGAGGTCTGGACGGCGGCAGGCGGCAAGGACTATGCGGGCAAGCCGCGCCCCGTCGTCATCGTGCAGGA
CGACAGCTTTGACGCGACGAACTCCATCACTGTCTGCGCCTTCACGACCGACGAAACCGAGGCGCCACTTTTCCGGCTCA
AGGTCGAGCCGAACGACGGCAACGGCCTTCGAGCTCCGTCTCGACTTATGGTCGACAAGATTACTACGGTGCCAAAAGCC
AAGGTAGGAACGTTAGTGGGACGCCTAGACGATGACGACCTGCTGCGTCTCAATCGCGCTATTCTGGTGTTTCTCGGACT
GGCTGGTTCGCAGAGAAGCGCCAGTGCAGATTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT197567 WP_001096429.1 NZ_CP072272:c64264-63965 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT197567 NZ_CP096255:7594421-7594642 [Bradyrhizobium barranii subsp. apii]
ATGAGCCCTTCAAAGCCCAAGCCGTCTCGTATGAAGGTGCAGGCCCACCGTGATCGATTGCGTGCACAAGGCCTGCGGCC
GATCCAGATCTGGGTGCCGGATATCCGCTCGCCCTCCTTCCAATCGGAAGCCCATCGCCAATCGCTTGCGGTGGCAACGA
GCTCACATGAATCCGAAGACCAAGGCTTCATTGATGCCGTATCCGATTGGACCGATGAATGA