Detailed information of TA system TA197562

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 14416..14909
• Replicon: plasmid pXEA29.3836R
• Accession: NZ_CP072269
• Organism: Xanthomonas euvesicatoria pv. alfalfae strain CFBP3836

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: XeaCFBP3836p_RS21625
• Protein ID: WP_015462614.1
• Coordinates: 14416..14742 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: XeaCFBP3836p_RS21630
• Protein ID: WP_017157755.1
• Coordinates: 14739..14909 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XeaCFBP3836p_RS21615 (XeaCFBP3836p_21615)	9796..11370	+	1575	WP_029820899.1	type IV secretion system DNA-binding domain-containing protein	-
XeaCFBP3836p_RS21620 (XeaCFBP3836p_21620)	11384..14359	+	2976	WP_209273136.1	MobF family relaxase	-
XeaCFBP3836p_RS21625 (XeaCFBP3836p_21625)	14416..14742	-	327	WP_015462614.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
XeaCFBP3836p_RS21630 (XeaCFBP3836p_21630)	14739..14909	-	171	WP_017157755.1	antitoxin MazE family protein	Antitoxin
XeaCFBP3836p_RS21635 (XeaCFBP3836p_21635)	15134..15529	-	396	WP_017172645.1	type II toxin-antitoxin system VapC family toxin	-
XeaCFBP3836p_RS21640 (XeaCFBP3836p_21640)	15526..15777	-	252	WP_003108276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
XeaCFBP3836p_RS21645 (XeaCFBP3836p_21645)	15956..16528	+	573	WP_029820909.1	recombinase family protein	-
XeaCFBP3836p_RS21650 (XeaCFBP3836p_21650)	16682..17041	-	360	WP_029820911.1	hypothetical protein	-
XeaCFBP3836p_RS21655 (XeaCFBP3836p_21655)	17096..17671	-	576	WP_029820912.1	phospholipase D family protein	-
XeaCFBP3836p_RS21660 (XeaCFBP3836p_21660)	17746..18633	-	888	WP_029820914.1	lytic transglycosylase domain-containing protein	-
XeaCFBP3836p_RS21665 (XeaCFBP3836p_21665)	18644..19666	-	1023	WP_011052983.1	P-type DNA transfer ATPase VirB11	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..28841	28841
	-	flank	IS/Tn	-	-	15956..16528	572

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (2-54)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11676.70 Da
• Isoelectric Point: 9.2550

>T197562 WP_015462614.1 NZ_CP072269:c14742-14416 [Xanthomonas euvesicatoria pv. alfalfae]
MMRGDFVTIAMQGDFGKPRPALVIQADQFDAHTTVTVLPVTSTLVAAPLLRITVHPSTDNGLQKPSQVMVDKAMTVKRDK
VGRAFGRVDADALVEIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T197562 NZ_CP096245:6225-6539 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6518.34 Da
• Isoelectric Point: 4.1476

>AT197562 WP_017157755.1 NZ_CP072269:c14909-14739 [Xanthomonas euvesicatoria pv. alfalfae]
MAGLRPVQIWVPDTRRPDFAEECRRQCRLAAQADMADTDMQRFMDEALADMDGWTE

Nucleotide

• Length: 171 bp

>AT197562 NZ_CP096245:5959-6237 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA