Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 246792..247434 Replicon chromosome
Accession NZ_CP071811
Organism Bacillus mycoides strain JAS15/1

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag EXW36_RS01380 Protein ID WP_000635965.1
Coordinates 247084..247434 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EXW36_RS01375 Protein ID WP_000004570.1
Coordinates 246792..247079 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW36_RS01350 (EXW36_01350) 242108..243070 + 963 WP_002009966.1 UV DNA damage repair endonuclease UvsE -
EXW36_RS01355 (EXW36_01355) 243063..243635 - 573 WP_002167128.1 rhomboid family intramembrane serine protease -
EXW36_RS01360 (EXW36_01360) 243728..244087 + 360 WP_002009969.1 holo-ACP synthase -
EXW36_RS01365 (EXW36_01365) 244244..245194 + 951 WP_002091373.1 outer membrane lipoprotein carrier protein LolA -
EXW36_RS01370 (EXW36_01370) 245312..246481 + 1170 WP_016126474.1 alanine racemase -
EXW36_RS01375 (EXW36_01375) 246792..247079 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EXW36_RS01380 (EXW36_01380) 247084..247434 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EXW36_RS01385 (EXW36_01385) 247503..249671 + 2169 WP_002029442.1 Tex family protein -
EXW36_RS01390 (EXW36_01390) 249729..249845 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EXW36_RS01395 (EXW36_01395) 250056..250511 + 456 WP_016095131.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T196974 WP_000635965.1 NZ_CP071811:247084-247434 [Bacillus mycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T196974 NZ_CP095520:c2368207-2368105 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT196974 WP_000004570.1 NZ_CP071811:246792-247079 [Bacillus mycoides]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT196974 NZ_CP095520:2368067-2368335 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References