Detailed information of TA system TA196816

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-pasA/RelE-HTH
• Location: 2199678..2200167
• Replicon: chromosome
• Accession: NZ_CP071733
• Organism: Bifidobacterium longum subsp. infantis strain JCM 7010

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BLI010_RS09740
• Protein ID: WP_229773994.1
• Coordinates: 2199940..2200167 (+)
• Length: 76 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: A0A4R0VXI9
• Locus tag: BLI010_RS09735
• Protein ID: WP_060620048.1
• Coordinates: 2199678..2199914 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLI010_RS09720 (BLI010_09720)	2195831..2197018	+	1188	WP_206648675.1	carboxylate--amine ligase	-
BLI010_RS09725 (BLI010_09725)	2197181..2198371	+	1191	WP_206648674.1	MFS transporter	-
BLI010_RS09730 (BLI010_09730)	2198454..2199095	+	642	WP_158082776.1	4'-phosphopantetheinyl transferase superfamily protein	-
BLI010_RS09735 (BLI010_09735)	2199678..2199914	+	237	WP_060620048.1	ribbon-helix-helix domain-containing protein	Antitoxin
BLI010_RS09740 (BLI010_09740)	2199940..2200167	+	228	WP_229773994.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BLI010_RS09745 (BLI010_09745)	2200518..2201180	-	663	WP_131223048.1	tRNA (cytidine(34)-2'-O)-methyltransferase	-
BLI010_RS09750 (BLI010_09750)	2201201..2202157	+	957	WP_032744820.1	A/G-specific adenine glycosylase	-
BLI010_RS09755 (BLI010_09755)	2202222..2202944	+	723	WP_060620046.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2191567..2200167	8600

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-72)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 76 a.a.
• Molecular weight: 8653.22 Da
• Isoelectric Point: 10.1818

>T196816 WP_229773994.1 NZ_CP071733:2199940-2200167 [Bifidobacterium longum subsp. infantis]
MKKLDRNVAKRIIAKLREISQLEDPRSTGKALAGNLAGLWRYRVGDYRIVCDIEDEVLLILVIDVAHRSKVYKRC

Nucleotide

• Length: 228 bp

>T196816 NZ_CP095428:4587170-4587655 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8970.23 Da
• Isoelectric Point: 4.2821

>AT196816 WP_060620048.1 NZ_CP071733:2199678-2199914 [Bifidobacterium longum subsp. infantis]
MATVTAALRMPVELAARYDCLAKATGRTKTFYMNEALTESIDRFEYEYGIMKKVEDWRAGRLETVTLDELEESLVLED

Nucleotide

• Length: 237 bp

>AT196816 NZ_CP095428:4586880-4587173 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A4R0VXI9