Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 83884..84527 Replicon plasmid pL3
Accession NZ_CP071714
Organism Escherichia coli strain EC20017429

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag JQM62_RS29890 Protein ID WP_001044768.1
Coordinates 84111..84527 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag JQM62_RS29885 Protein ID WP_001261287.1
Coordinates 83884..84114 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JQM62_RS29855 (JQM62_29615) 79749..81188 + 1440 WP_001213545.1 enterohemolysin T1SS ABC transporter subunit EhxD -
JQM62_RS29860 (JQM62_29620) 81255..81398 + 144 Protein_76 transcriptional regulator -
JQM62_RS29865 (JQM62_29625) 81402..81593 + 192 Protein_77 lipid II-degrading bacteriocin -
JQM62_RS29870 (JQM62_29630) 81601..81954 - 354 WP_000864810.1 colicin M immunity protein -
JQM62_RS29875 (JQM62_29635) 82127..82909 - 783 WP_001164205.1 site-specific integrase -
JQM62_RS29880 (JQM62_29640) 82911..83324 - 414 WP_000465041.1 hypothetical protein -
JQM62_RS29885 (JQM62_29645) 83884..84114 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
JQM62_RS29890 (JQM62_29650) 84111..84527 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
JQM62_RS29895 (JQM62_29655) 84689..85234 - 546 WP_001165114.1 hypothetical protein -
JQM62_RS29900 (JQM62_29660) 85302..86458 + 1157 WP_085948186.1 IS3-like element IS600 family transposase -
JQM62_RS29905 (JQM62_29665) 87263..88123 - 861 WP_000704534.1 alpha/beta hydrolase -
JQM62_RS29910 (JQM62_29670) 88251..88607 + 357 Protein_86 tyrosine-type recombinase/integrase -
JQM62_RS29915 (JQM62_29675) 88691..89365 + 675 WP_001341423.1 IS66-like element accessory protein TnpA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - toxB / toxB / toxB / toxB / espP / hlyC / hlyC / hlyA / hlyB / hlyD 1..94094 94094


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T196784 WP_001044768.1 NZ_CP071714:84111-84527 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T196784 NZ_CP095421:c4944360-4944076 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT196784 WP_001261287.1 NZ_CP071714:83884-84114 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT196784 NZ_CP095421:c4944592-4944350 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References