Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1757410..1757951 Replicon chromosome
Accession NZ_CP071711
Organism Escherichia coli strain EC20017429

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag JQM62_RS09005 Protein ID WP_000615976.1
Coordinates 1757673..1757951 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag JQM62_RS09000 Protein ID WP_000729704.1
Coordinates 1757410..1757670 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JQM62_RS08975 (1753193) 1753193..1753978 - 786 WP_000207567.1 putative lateral flagellar export/assembly protein LafU -
JQM62_RS08980 (1753950) 1753950..1755662 + 1713 Protein_1771 flagellar biosynthesis protein FlhA -
JQM62_RS08985 (1755767) 1755767..1756045 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
JQM62_RS08990 (1756038) 1756038..1756394 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
JQM62_RS08995 (1756451) 1756451..1757224 - 774 WP_000543899.1 C40 family peptidase -
JQM62_RS09000 (1757410) 1757410..1757670 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JQM62_RS09005 (1757673) 1757673..1757951 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JQM62_RS09010 (1758107) 1758107..1758847 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
JQM62_RS09015 (1758818) 1758818..1759585 - 768 WP_000333380.1 class II glutamine amidotransferase -
JQM62_RS09020 (1759791) 1759791..1760369 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T196759 WP_000615976.1 NZ_CP071711:1757673-1757951 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T196759 NZ_CP095403:c183236-182955 [Rhodococcus opacus]
GTGATCGAGTCGTTCGCCGACAAAGAGACCGAGCGGGTCTTTCACCGACAGTTCTCGAAGAAGCTCCCGCAGGACATTCA
GCGGATCGCGCGCCGGCGCCTCCTGTTGATCGAGGCTGCCACCGACGTCAACGACCTGAGGGTGCCCCCAGGCAACCGCC
TGGAGAAGCTCTCCGGGGACCGGGTGGGCCAGTACAGCGTTCGGATCAATGATCAGTACCGCATCTGTTTCATCTGGCGC
GCCACCGCCGCATACGACGTGGAGATCACCGACTATCACTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT196759 WP_000729704.1 NZ_CP071711:1757410-1757670 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT196759 NZ_CP095403:c182896-182603 [Rhodococcus opacus]
ATGACTACAGACCTGAGCCCAATTCACCCCGGCGAGATCCTCCGCGAGGACTTCCTCGAGCCGTTCGGCATCACGCAGAA
CCGCCTAGCCACGATCATCGGTGTTCCGCCCCGACGGATCAACGAGATCGTGCTCGGCAAGCGGTCGATCACCGCAGACA
CCGCGTTGCGGCTGGCCAAGGCGTTCGGCAACAGTGCGCAGTTCTGGCTCAATATCCAGGACCGCTACGAGCTCGACCTT
GCGGCCGACAAGATAGGCGACGCCCTCGCCAAGATCACGCGTCTCTCGGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References