Detailed information of TA system TA196619

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 240506..241191
• Replicon: plasmid p5
• Accession: NZ_CP071623
• Organism: Rhizobium bangladeshense strain PLR8-1a

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J2J97_RS30430
• Protein ID: WP_207609802.1
• Coordinates: 240769..241191 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1C3Y1G2
• Locus tag: J2J97_RS30425
• Protein ID: WP_064709306.1
• Coordinates: 240506..240772 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J2J97_RS30395	235652..236116	-	465	WP_064709300.1	Lrp/AsnC family transcriptional regulator	-
J2J97_RS30400	236278..237057	+	780	WP_207609797.1	ATP-binding cassette domain-containing protein	-
J2J97_RS30405	237106..237879	+	774	WP_207609798.1	transporter substrate-binding domain-containing protein	-
J2J97_RS30410	237892..238617	+	726	WP_207609799.1	ABC transporter permease subunit	-
J2J97_RS30415	238631..239320	+	690	WP_207609800.1	ABC transporter permease subunit	-
J2J97_RS30420	239397..240305	+	909	WP_207609801.1	homocysteine S-methyltransferase family protein	-
J2J97_RS30425	240506..240772	+	267	WP_064709306.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
J2J97_RS30430	240769..241191	+	423	WP_207609802.1	PIN domain-containing protein	Toxin
J2J97_RS30435	241268..242038	-	771	WP_064712938.1	ABC transporter ATP-binding protein	-
J2J97_RS30440	242048..243031	-	984	WP_207609803.1	ABC transporter substrate-binding protein	-
J2J97_RS30445	243070..243921	-	852	WP_064709310.1	ABC transporter permease	-
J2J97_RS30450	243932..245284	-	1353	WP_207609804.1	NtaA/DmoA family FMN-dependent monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..284792	284792

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-60)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15444.69 Da
• Isoelectric Point: 4.9493

>T196619 WP_207609802.1 NZ_CP071623:240769-241191 [Rhizobium bangladeshense]
VRLLLDTNVLSEVTKPNPDVRVLGWLGGLDEDLSFISVVSVAEIRRGVTLMDNGRKRDVLADWLARDLPQRFERRIIPVD
EAVALAWGDLMGHAKRNGRGLSSMDGLIAATAIAHDLTLATRNTRDFEGFGIELVDPWVG

Nucleotide

• Length: 423 bp

>T196619 NZ_CP095186:c2189007-2188813 [Pseudoxanthomonas mexicana]
GTGCCACCCAAGATTCGCGATGTGTTGCAACAACTCAAGGACGCGGGCTGGCAGCAGGTGGGCCAGACCGGCAGCCACCG
GCAGTTCAAGCACCCCACGCTTCCAGGCCGCGTGACAGTCGCCGGCAAGCCGGGTGATGATGTGGCGCCCGGGACATTGG
CCAGCATCCTGAAGCAGGCCGGGATCAAGAGGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9781.96 Da
• Isoelectric Point: 6.3615

>AT196619 WP_064709306.1 NZ_CP071623:240506-240772 [Rhizobium bangladeshense]
MQSSRQGDESWTVASAKAKLSEVIERAQSAPQTITRNGKPSVVVVSAEEWQRKIARKGTLAEFLMESPLRGADLDLERQR
DEPRDSGL

Nucleotide

• Length: 267 bp

>AT196619 NZ_CP095186:c2188807-2188595 [Pseudoxanthomonas mexicana]
ATGCGCTACGCCATCGTCATCGAGCAAGCTGGCGGCAACTATTCCGCGTATGTCCCGGACCTGCCGGGTTGCGTGGCCAC
CGGTGCCACCAAGGCCGAGGTGGAAGTACTGATCCACGACGCCATTGCGTTGCATCTGGCCGGATTGCGCGAGGACGGCG
TGGCCATTCCGGTGCCTTCGAGCCAGGTGGAGTACATCGACGTCGCGGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A1C3Y1G2