Detailed information of TA system TA196618

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 166073..166680
• Replicon: plasmid p4
• Accession: NZ_CP071622
• Organism: Rhizobium bangladeshense strain PLR8-1a

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: J0VFB1
• Locus tag: J2J97_RS28400
• Protein ID: WP_003572077.1
• Coordinates: 166426..166680 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J2J97_RS28395
• Protein ID: WP_054185827.1
• Coordinates: 166073..166429 (-)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J2J97_RS28380	161279..162967	+	1689	WP_207609750.1	GMC family oxidoreductase N-terminal domain-containing protein	-
J2J97_RS28385	162992..165505	+	2514	WP_207609728.1	CoA transferase	-
J2J97_RS28390	165622..166035	+	414	Protein_155	ABC transporter substrate-binding protein	-
J2J97_RS28395	166073..166429	-	357	WP_054185827.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
J2J97_RS28400	166426..166680	-	255	WP_003572077.1	type II toxin-antitoxin system HicA family toxin	Toxin
J2J97_RS28405	167050..168084	-	1035	Protein_158	IS110 family transposase	-
J2J97_RS28410	168890..169736	-	847	Protein_159	alpha/beta hydrolase	-
J2J97_RS28415	169950..170654	+	705	WP_207609479.1	carbonic anhydrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..337471	337471
	-	flank	IS/Tn	-	-	167584..168084	500

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9336.70 Da
• Isoelectric Point: 9.8124

>T196618 WP_003572077.1 NZ_CP071622:c166680-166426 [Rhizobium bangladeshense]
MNSKQRKTLAAVFKDPVSGTIEWAAIENLLVAAGADLIEGNGSRVKFEKDGIIASFHRPHPDKEAKRYQVRDAREFLMQI
GVTP

Nucleotide

• Length: 255 bp

>T196618 NZ_CP095181:c142340-141924 [Enterobacter roggenkampii]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACCGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTAAGTAA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13288.93 Da
• Isoelectric Point: 4.9838

>AT196618 WP_054185827.1 NZ_CP071622:c166429-166073 [Rhizobium bangladeshense]
MNIMTYNGYHARIEFDAEDDVFFGRIAGISDVIGFHGDSVAELKKAFHEAVDDYLETCRKIGKEPQRPYSGKMMFRVAPE
VHRRAALAAELSGKSLNQWAEEVLEEAADHYAEARLPA

Nucleotide

• Length: 357 bp

>AT196618 NZ_CP095181:c142567-142337 [Enterobacter roggenkampii]
ATGCGCACAGTATCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTAG
ACAGGGCCGATCCGGACTTTATGGCGCAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGATCCATGA

Structures

Toxin

• AlphaFold DB: A0A246TAE7