Detailed information of TA system TA196481

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2076086..2077635
• Replicon: chromosome
• Accession: NZ_CP071490
• Organism: Glaesserella parasuis strain GHP1808

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: J1G53_RS10460
• Protein ID: WP_075592260.1
• Coordinates: 2076086..2077402 (-)
• Length: 439 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: U4SAZ6
• Locus tag: J1G53_RS10465
• Protein ID: WP_010786623.1
• Coordinates: 2077396..2077635 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1G53_RS10420	2071186..2071773	+	588	WP_075592760.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
J1G53_RS10425	2072029..2072208	-	180	Protein_2001	disulfide bond formation protein DsbA	-
J1G53_RS10430	2072208..2072651	-	444	WP_015939865.1	nuclear transport factor 2 family protein	-
J1G53_RS10435	2072786..2072902	+	117	Protein_2003	LysR family transcriptional regulator	-
J1G53_RS10440	2072983..2073556	-	574	Protein_2004	virulence RhuM family protein	-
J1G53_RS10445	2073743..2074102	-	360	WP_010787368.1	hypothetical protein	-
J1G53_RS10450	2074002..2074517	+	516	WP_080513259.1	hypothetical protein	-
J1G53_RS10455	2074541..2076082	+	1542	WP_202113897.1	PLP-dependent aminotransferase family protein	-
J1G53_RS10460	2076086..2077402	-	1317	WP_075592260.1	type II toxin-antitoxin system HipA family toxin	Toxin
J1G53_RS10465	2077396..2077635	-	240	WP_010786623.1	helix-turn-helix domain-containing protein	Antitoxin
J1G53_RS10470	2077783..2078610	-	828	WP_075592259.1	alpha/beta hydrolase	-
J1G53_RS10475	2078741..2080186	+	1446	WP_075592258.1	metalloprotease TldD	-
J1G53_RS10480	2080441..2080851	+	411	WP_010786620.1	preprotein translocase subunit SecE	-
J1G53_RS10485	2080853..2081431	+	579	WP_020457616.1	transcription termination/antitermination protein NusG	-
J1G53_RS10490	2081557..2081811	-	255	WP_005713300.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-104)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-66)

Sequences

Toxin

Protein

• Length: 439 a.a.
• Molecular weight: 49995.30 Da
• Isoelectric Point: 7.6047

>T196481 WP_075592260.1 NZ_CP071490:c2077402-2076086 [Glaesserella parasuis]
MVMRLRVLMNGEMVGYFDRTLSGSHHFTYAESWLNSPLARPISLSMPLQRATYQGKEVINFFDNLLPDNPQIRARIATRF
NAETQQPFDLLAKIGRDAAGAITLLGEDEQIPNHRIIQATELNESEMAKILQSYQYNVPLGMIDNNQDFRISIAGAQEKT
ALLWHNGKWKLPHGLTPTTHIFKLPMGEIRTPKHLLDLSKSVDNELVCLRLFSAFNIPSANVELLEMNDIRALAVERFDR
RLSQDGSYFLRLPYEDFCQAFGLSPTQKYESDGGIGIGDMMNKLLYSSNPNDRRQFMQTQFLFWLLAAIDGHAKNFSLAI
EKQGRYHLSPVYDVLSAYPMLGGKGWHHSELQMAMSLRSTQKGKKWHWSKIYAEHFYATAKFVGFSSSDMKQVIEQICDT
VQETIDSVATTLPKQIDENVRDQIFTGIMKQVQKVSMK

Nucleotide

• Length: 1317 bp

>T196481 NZ_CP095137:c3594007-3593705 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9059.47 Da
• Isoelectric Point: 9.7684

>AT196481 WP_010786623.1 NZ_CP071490:c2077635-2077396 [Glaesserella parasuis]
MLITTPDMLGNSLRDIRKQKKLSQMQIANQVALRQATISTFEKKTDKATIETLFKLLSGLGYELHIQPRPEKTEGNDEW

Nucleotide

• Length: 240 bp

>AT196481 NZ_CP095137:c3593669-3593328 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Antitoxin

• AlphaFold DB: U4SAZ6