Detailed information of TA system TA196422

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 3649854..3650674
• Replicon: chromosome
• Accession: NZ_CP071441
• Organism: Escherichia coli strain EcPF20

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: J1F34_RS17410
• Protein ID: WP_001054379.1
• Coordinates: 3650417..3650674 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: J1F34_RS17405
• Protein ID: WP_000123957.1
• Coordinates: 3649854..3650405 (-)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1F34_RS17375	3645548..3646861	-	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-
J1F34_RS17380	3646873..3647151	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
J1F34_RS17385	3647148..3648269	-	1122	WP_000010833.1	M42 family metallopeptidase	-
J1F34_RS17390	3648514..3648630	-	117	Protein_3386	VOC family protein	-
J1F34_RS17395	3648668..3648928	-	261	WP_113976402.1	hypothetical protein	-
J1F34_RS17400	3649056..3649802	-	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
J1F34_RS17405	3649854..3650405	-	552	WP_000123957.1	N-acetyltransferase	Antitoxin
J1F34_RS17410	3650417..3650674	-	258	WP_001054379.1	YjhX family toxin	Toxin
J1F34_RS17415	3651051..3651350	-	300	WP_001332034.1	GNAT family N-acetyltransferase	-
J1F34_RS17420	3651428..3652654	+	1227	Protein_3392	helicase YjhR	-
J1F34_RS17425	3653237..3654217	-	981	WP_000991462.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
J1F34_RS17430	3654281..3655387	-	1107	WP_001295733.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T196422 WP_001054379.1 NZ_CP071441:c3650674-3650417 [Escherichia coli]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T196422 NZ_CP095121:3915371-3915589 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT196422 WP_000123957.1 NZ_CP071441:c3650405-3649854 [Escherichia coli]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT196422 NZ_CP095121:3914970-3915344 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: B6I030