Detailed information of TA system TA196250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 72389..73034
• Replicon: chromosome
• Accession: NZ_CP071383
• Organism: Leclercia sp. 4-9-1-25

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: JZ655_RS00350
• Protein ID: WP_207293795.1
• Coordinates: 72672..73034 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JZ655_RS00345
• Protein ID: WP_207292719.1
• Coordinates: 72389..72688 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JZ655_RS00320	67594..68130	+	537	WP_040077805.1	hypothetical protein	-
JZ655_RS00325	68147..69976	+	1830	WP_207292716.1	dynamin family protein	-
JZ655_RS00330	69978..71627	+	1650	WP_207292717.1	50S ribosome-binding GTPase	-
JZ655_RS00335	71624..71887	+	264	WP_040077809.1	YkgJ family cysteine cluster protein	-
JZ655_RS00340	71934..72374	+	441	WP_207292718.1	GNAT family N-acetyltransferase	-
JZ655_RS00345	72389..72688	-	300	WP_207292719.1	XRE family transcriptional regulator	Antitoxin
JZ655_RS00350	72672..73034	-	363	WP_207293795.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JZ655_RS00360	73722..75113	+	1392	WP_046886314.1	glycoside-pentoside-hexuronide family transporter	-
JZ655_RS00365	75126..77444	+	2319	WP_207292720.1	alpha-xylosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13776.90 Da
• Isoelectric Point: 8.9064

>T196250 WP_207293795.1 NZ_CP071383:c73034-72672 [Leclercia sp. 4-9-1-25]
MWTILLGAHFEQWLNAQDEGLQEKVLAALLNLQVYGPRLPRPYADTVKGSQHKNMKELRIQYAGRPVRALFAFDPLRRAI
VLCAGDKSNDKRFYDNLIRIADEAFSLHITVLETLNENPK

Nucleotide

• Length: 363 bp

>T196250 NZ_CP095023:c2853215-2852796 [Mycobacterium tuberculosis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11132.93 Da
• Isoelectric Point: 5.8669

>AT196250 WP_207292719.1 NZ_CP071383:c72688-72389 [Leclercia sp. 4-9-1-25]
MKTLNEAIASRSPESQQRIQQMADEMILETGLQLVREELNLSQKQLAELMGISQPAITQLEQRGNELKIATLKRYIEAMG
GKLSIDVELPTGKRIAFHI

Nucleotide

• Length: 300 bp

>AT196250 NZ_CP095023:c2853436-2853212 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA