Detailed information of TA system TA196219

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2117269..2117494
• Replicon: chromosome
• Accession: NZ_CP071375
• Organism: Escherichia coli strain E105

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: J0O73_RS10080
• Protein ID: WP_000813258.1
• Coordinates: 2117339..2117494 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2117269..2117327 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0O73_RS10040	2112696..2113160	+	465	Protein_1943	phage replisome organizer	-
J0O73_RS10045	2113773..2114519	+	747	WP_000788759.1	ATP-binding protein	-
J0O73_RS10050	2114541..2115302	+	762	WP_000451006.1	DUF1627 domain-containing protein	-
J0O73_RS10055	2115335..2115616	+	282	WP_042353845.1	DNA-binding protein	-
J0O73_RS10060	2115613..2115840	+	228	WP_000699809.1	hypothetical protein	-
J0O73_RS10065	2115833..2116168	+	336	WP_001365112.1	hypothetical protein	-
J0O73_RS10070	2116271..2116489	+	219	WP_000683607.1	DUF4014 family protein	-
J0O73_RS10075	2116491..2117048	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2117269..2117327	-	59	-	-	Antitoxin
J0O73_RS10080	2117339..2117494	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
J0O73_RS10085	2117614..2117958	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
J0O73_RS10090	2118080..2118352	+	273	WP_000191872.1	hypothetical protein	-
J0O73_RS10095	2118354..2119403	+	1050	WP_001265233.1	DUF968 domain-containing protein	-
J0O73_RS10100	2119416..2119790	+	375	WP_001217416.1	RusA family crossover junction endodeoxyribonuclease	-
J0O73_RS10105	2119787..2120608	+	822	WP_000762928.1	antitermination protein	-
J0O73_RS10120	2121174..2121605	+	432	WP_207184260.1	TerB family tellurite resistance protein	-
J0O73_RS10125	2121602..2121857	+	256	Protein_1958	DUF3927 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2105971..2150177	44206

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T196219 WP_000813258.1 NZ_CP071375:2117339-2117494 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T196219 NZ_CP095023:704786-705187 [Mycobacterium tuberculosis]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin

Nucleotide

• Length: 59 bp

>AT196219 NZ_CP071375:c2117327-2117269 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin