Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 2767148..2767760 Replicon chromosome
Accession NZ_CP071263
Organism Escherichia coli strain MEI003

Toxin (Protein)


Gene name hicA Uniprot ID U9YXE2
Locus tag J0G23_RS13600 Protein ID WP_000833473.1
Coordinates 2767575..2767760 (-) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1V3VHB3
Locus tag J0G23_RS13595 Protein ID WP_000499746.1
Coordinates 2767148..2767558 (-) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J0G23_RS13580 (2762660) 2762660..2763475 - 816 WP_000891838.1 AraC family transcriptional regulator -
J0G23_RS13585 (2763701) 2763701..2765101 + 1401 WP_000204800.1 MFS transporter -
J0G23_RS13590 (2765112) 2765112..2767076 + 1965 WP_024230365.1 glycoside hydrolase family 127 protein -
J0G23_RS13595 (2767148) 2767148..2767558 - 411 WP_000499746.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
J0G23_RS13600 (2767575) 2767575..2767760 - 186 WP_000833473.1 type II toxin-antitoxin system HicA family toxin Toxin
J0G23_RS13605 (2768235) 2768235..2769314 + 1080 WP_000061477.1 type I restriction enzyme HsdR N-terminal domain-containing protein -
J0G23_RS13610 (2769355) 2769355..2770893 - 1539 WP_000183978.1 aldehyde dehydrogenase AldB -
J0G23_RS13615 (2771094) 2771094..2772245 - 1152 WP_000741508.1 L-threonine dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6800.89 Da        Isoelectric Point: 11.7053

>T196106 WP_000833473.1 NZ_CP071263:c2767760-2767575 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Download         Length: 186 bp

>T196106 NZ_CP095021:716662-717069 [Mycobacterium tuberculosis]
GTGAGCACGACGCCGGCCGCCGGAGTGCTCGACACGTCGGTGTTCATCGCGACCGAAAGCGGCCGGCAACTCGACGAGGC
GCTGATCCCCGACCGGGTCGCCACCACCGTCGTCACCCTCGCCGAACTGCGCGTCGGCGTGCTGGCCGCGGCGACGACCG
ACATCCGGGCTCAACGCCTGGCGACCCTGGAATCCGTTGCCGATATGGAAACGTTGCCCGTCGACGACGATGCCGCCCGA
ATGTGGGCCCGATTGCGGATCCATCTTGCCGAGTCCGGTCGCCGGGTGCGGATCAACGACCTGTGGATCGCGGCCGTCGC
GGCATCGCGAGCGCTGCCGGTCATCACCCAGGACGACGACTTCGCCGCCCTCGACGGTGCGGCCAGTGTGGAGATCATTC
GGGTCTGA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15290.22 Da        Isoelectric Point: 4.5486

>AT196106 WP_000499746.1 NZ_CP071263:c2767558-2767148 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDYAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Download         Length: 411 bp

>AT196106 NZ_CP095021:716405-716665 [Mycobacterium tuberculosis]
ATGTCTGAGGTGGCCTCGCGTGAGCTGCGTAACGATACGGCCGGCGTGCTGCGCCGCGTGCGGGCAGGGGAGGACGTCAC
CATCACCGTCAGCGGCCGTCCGGTCGCGGTGCTTACCCCGGTTCGTCCGCGGCGCCGGCGTTGGCTGAGCAAAACGGAGT
TCCTGTCGCGGTTGCGCGGCGCTCAAGCCGATCCCGGGCTCCGTAACGACCTCGCGGTCCTTGCCGGCGACACGACCGAG
GATCTCGGGCCGATCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A1V3VHB3

References