Detailed information of TA system TA196082

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 450224..450449
• Replicon: chromosome
• Accession: NZ_CP071263
• Organism: Escherichia coli strain MEI003

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: J0G23_RS02285
• Protein ID: WP_000813254.1
• Coordinates: 450224..450379 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	450391..450449 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0G23_RS02250	445266..445478	+	213	WP_000087756.1	cold shock-like protein CspF	-
J0G23_RS02255	445533..445622	+	90	WP_120795389.1	hypothetical protein	-
J0G23_RS02260	446248..446433	+	186	WP_000203370.1	hypothetical protein	-
J0G23_RS02265	447594..448346	-	753	WP_001047121.1	antitermination protein	-
J0G23_RS02270	448360..449409	-	1050	WP_001531701.1	DUF968 domain-containing protein	-
J0G23_RS02275	449411..449689	-	279	WP_023147795.1	hypothetical protein	-
J0G23_RS02280	449756..450007	-	252	WP_000980999.1	protein Rem	-
J0G23_RS02285	450224..450379	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	450391..450449	+	59	-	-	Antitoxin
J0G23_RS02290	450995..451660	-	666	WP_021568046.1	epoxyqueuosine reductase QueH	-
J0G23_RS02295	451771..451947	-	177	Protein_460	methyltransferase	-
J0G23_RS02300	451944..452366	-	423	WP_001151183.1	DUF977 family protein	-
J0G23_RS02305	452407..453372	-	966	WP_021568047.1	hypothetical protein	-
J0G23_RS02310	453353..453874	-	522	WP_250375858.1	toxin YdaT family protein	-
J0G23_RS02315	453858..454085	-	228	WP_000476994.1	Cro/CI family transcriptional regulator	-
J0G23_RS02320	454163..454570	+	408	WP_024239371.1	helix-turn-helix domain-containing protein	-
J0G23_RS02325	454763..454918	+	156	WP_000379575.1	DUF1391 family protein	-
J0G23_RS25735	454920..455021	+	102	Protein_467	protein YdfB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	416047..460562	44515

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T196082 WP_000813254.1 NZ_CP071263:c450379-450224 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T196082 NZ_CP095020:c3064103-3063687 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT196082 NZ_CP071263:450391-450449 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTCTAGCATGAAATTGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin