Detailed information of TA system TA195819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4453991..4454586
• Replicon: chromosome
• Accession: NZ_CP071222
• Organism: Serratia marcescens subsp. marcescens ATCC 13880 substr. Sm_S28_jyu2015

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1S6LRI1
• Locus tag: J0F81_RS21245
• Protein ID: WP_071605322.1
• Coordinates: 4454332..4454586 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J0F81_RS21240
• Protein ID: WP_033633573.1
• Coordinates: 4453991..4454332 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0F81_RS21220	4449499..4450314	-	816	WP_033639337.1	GPO family capsid scaffolding protein	-
J0F81_RS21225	4450457..4452229	+	1773	WP_033639336.1	terminase ATPase subunit family protein	-
J0F81_RS21230	4452229..4452915	+	687	WP_060445964.1	terminase family protein	-
J0F81_RS21235	4452912..4453946	+	1035	WP_033639335.1	phage portal protein	-
J0F81_RS21240	4453991..4454332	-	342	WP_033633573.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
J0F81_RS21245	4454332..4454586	-	255	WP_071605322.1	type II toxin-antitoxin system HicA family toxin	Toxin
J0F81_RS21250	4454956..4455153	-	198	WP_126186641.1	hypothetical protein	-
J0F81_RS21255	4455201..4455425	-	225	WP_033639333.1	hypothetical protein	-
J0F81_RS21260	4455464..4457680	-	2217	WP_033639332.1	replication endonuclease	-
J0F81_RS21265	4457677..4457991	-	315	WP_033639331.1	DUF3850 domain-containing protein	-
J0F81_RS21270	4458005..4458178	-	174	WP_162531492.1	hypothetical protein	-
J0F81_RS21275	4458118..4458342	-	225	WP_033639330.1	TraR/DksA C4-type zinc finger protein	-
J0F81_RS21280	4458342..4458584	-	243	WP_023447523.1	DUF2732 family protein	-
J0F81_RS21285	4458648..4458950	-	303	WP_031221632.1	hypothetical protein	-
J0F81_RS21290	4458962..4459141	-	180	WP_072009648.1	DUF2724 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	rfaE	4413279..4462179	48900

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (55-100)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9495.97 Da
• Isoelectric Point: 11.1073

>T195819 WP_071605322.1 NZ_CP071222:c4454586-4454332 [Serratia marcescens subsp. marcescens ATCC 13880]
MNKRHQKTLSDVFARPVNGSIKWSDIEALFTALGAEIHEREGSRIAVLLKGEKRVFHRPHPRPTTDKGAVNSIRIWLDSL
GIKP

Nucleotide

• Length: 255 bp

>T195819 NZ_CP094851:c141221-140940 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12399.90 Da
• Isoelectric Point: 4.5789

>AT195819 WP_033633573.1 NZ_CP071222:c4454332-4453991 [Serratia marcescens subsp. marcescens ATCC 13880]
MNNTLKIDGHTAVINFDPEIEMFRGEFVGLNGGADFYAYSVDELKKEGAISLAVFLDECHKDGIEPYKSYSGKVTTRLSP
ERHQALAIAAQATGQSINELLNEGVDLVIEKHS

Nucleotide

• Length: 342 bp

>AT195819 NZ_CP094851:c141445-141218 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACATCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• AlphaFold DB: A0A1S6LRI1