Detailed information of TA system TA195450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 1162036..1162580
• Replicon: chromosome
• Accession: NZ_CP071137
• Organism: MAG: Ferrovum myxofaciens isolate MI1III

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JZL65_RS05745
• Protein ID: WP_031596839.1
• Coordinates: 1162036..1162362 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: JZL65_RS05750
• Protein ID: WP_082783138.1
• Coordinates: 1162359..1162580 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JZL65_RS05720 (JZL65_05750)	1157831..1158415	+	585	WP_273145884.1	chorismate lyase	-
JZL65_RS05725 (JZL65_05755)	1158494..1159135	+	642	WP_062187741.1	response regulator transcription factor	-
JZL65_RS05730 (JZL65_05760)	1159143..1161278	-	2136	WP_273145885.1	DUF748 domain-containing protein	-
JZL65_RS05735 (JZL65_05765)	1161282..1161434	-	153	WP_273145886.1	hypothetical protein	-
JZL65_RS05740 (JZL65_05770)	1161431..1161865	-	435	WP_150112405.1	cytochrome C	-
JZL65_RS05745 (JZL65_05775)	1162036..1162362	-	327	WP_031596839.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JZL65_RS05750 (JZL65_05780)	1162359..1162580	-	222	WP_082783138.1	antitoxin MazE family protein	Antitoxin
JZL65_RS05755 (JZL65_05785)	1162815..1163153	+	339	WP_031599590.1	toxin	-
JZL65_RS05760 (JZL65_05790)	1163150..1163464	+	315	WP_031599591.1	transcriptional regulator	-
JZL65_RS05765 (JZL65_05795)	1163970..1164191	-	222	WP_082783138.1	antitoxin MazE family protein	-
JZL65_RS05770 (JZL65_05800)	1164360..1164782	-	423	WP_031599221.1	type II toxin-antitoxin system VapC family toxin	-
JZL65_RS05775 (JZL65_05805)	1164779..1165093	-	315	WP_031599223.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
JZL65_RS05780 (JZL65_05810)	1165420..1167315	-	1896	WP_062187752.1	GGDEF domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-105)

Antitoxin

• Antitoxin MazE-like (7-71)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11683.81 Da
• Isoelectric Point: 9.2533

>T195450 WP_031596839.1 NZ_CP071137:c1162362-1162036 [Ferrovum myxofaciens]
MMRGDFVTIVMQGDFGKPRPALVIQADQFGEHATVTVLPMTSTLVAAPLLRTPIQPSAENGLNKPSQVMIDKAMTVKRDK
LGQAFGRIDGVVMTEVERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T195450 NZ_CP094725:1072352-1072504 [Staphylococcus felis]
ATGTATCAAGACAAAAAAGATTATGATAATGAAATGGTCGATGGTTTTGACGATATGGTAGAACTTGGTAAGGAGATGGA
ACAAATTTCTGAAGAAAATGACCAAGAAAAGCTCAATCAAGACCATGATCAAGCAGTGCGTTCTGATAAATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8652.76 Da
• Isoelectric Point: 5.5794

>AT195450 WP_082783138.1 NZ_CP071137:c1162580-1162359 [Ferrovum myxofaciens]
MATHVNTRVQRHRDTLRMAGLRPIQIWVPDTRRPDFAEECRRQSRLVAQTDMVDTDLQHFMDEALADMEGWTE

Nucleotide

• Length: 222 bp

>AT195450 NZ_CP094725:1071735-1072334 [Staphylococcus felis]
ATGGCAATGAATTTTAAAATTTTTAAAGATGCAGATACAGCAGCGCTTTATACAGCTGATATTATTAGAAAACAATTTAA
TAATAATCCGACTACAATCGCAGGTATTCACTTAAATAATGAAGATGCGCCAGTACTAGAAGCGTTGAAAAAAAACGTTG
ATGATCATGCGGTAGATTTTAGCCAAATTCATATTTTAGATTACGATAAAAAAACATCTTACTATAAAGCTTTAGGTGTG
CCAGAAAAACAAGTGCATGAAATTGCAGAAGAAGAGCAAGTGGAAAGTTTTATCGAGCATCATGCTAAAACAAAAAATAA
TAAAGGTAAATTAACGCTTCAAGTTGTGACAATAGATCAAAAAGGTTATCTTGGTGTAGGGATGAAAGAAGGTATACTCC
CTGCTCGTGAAATTATTCTAGTTATCACTGGTGACCATAAAGCAGATTTAGTAAAAAAACTATATGAAGAAAATGGGAAT
ACAAGTTTTATCCCATCGACACTTAAACAGCATAGAATGGTCAATGTGGTTTTAGATGAAGCTGCTGCACAAGGGCTACC
TGAAGATGTCCGTTCGTATTTCACTTCATTATATGCTTAA