Detailed information of TA system TA195418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1947027..1947640
• Replicon: chromosome
• Accession: NZ_CP071128
• Organism: Mycobacterium tuberculosis strain 267/47W148

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JZL63_RS08980
• Protein ID: WP_206281165.1
• Coordinates: 1947027..1947416 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ52
• Locus tag: JZL63_RS08985
• Protein ID: WP_003408469.1
• Coordinates: 1947413..1947640 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JZL63_RS08950	1942656..1943570	+	915	WP_003898985.1	3-hydroxyacyl-CoA dehydrogenase family protein	-
JZL63_RS08955	1943573..1944403	+	831	WP_206281164.1	cyclase family protein	-
JZL63_RS08960	1944403..1944753	+	351	WP_003898986.1	cupin domain-containing protein	-
JZL63_RS08965	1944806..1945624	+	819	WP_003408456.1	3-keto-5-aminohexanoate cleavage protein	-
JZL63_RS08970	1945638..1946417	+	780	WP_003408460.1	IclR family transcriptional regulator	-
JZL63_RS08980	1947027..1947416	-	390	WP_206281165.1	PIN domain-containing protein	Toxin
JZL63_RS08985	1947413..1947640	-	228	WP_003408469.1	antitoxin	Antitoxin
JZL63_RS08990	1947858..1949339	+	1482	Protein_1773	carboxylase	-
JZL63_RS08995	1949339..1950586	+	1248	WP_206281166.1	serine hydrolase	-
JZL63_RS09000	1950629..1951048	-	420	WP_206281167.1	hypothetical protein	-
JZL63_RS09005	1951038..1951748	-	711	WP_003408486.1	winged helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (3-117)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 13793.99 Da
• Isoelectric Point: 6.9779

>T195418 WP_206281165.1 NZ_CP071128:c1947416-1947027 [Mycobacterium tuberculosis]
VIVLDASAAVELMLTTPAGAAVARRLRGETVLAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Nucleotide

• Length: 390 bp

>T195418 NZ_CP094715:1923938-1924123 [Staphylococcus pasteuri]
ATGAGCTTACATTTCACTATTCTTTTTTGGTTATCAATTATATTTTTAATTGCAGGTACAATCGTTCTTGTTACTATGCT
TAAAACTAAAAAAGAATCTAAAAAAGAATCTTATCTTGGCTTCACAATTGTATTCTTTATATTTGGTTTAGCCATGTTAA
TTTATACATTAATATTCGGTTTGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8226.39 Da
• Isoelectric Point: 4.3313

>AT195418 WP_003408469.1 NZ_CP071128:c1947640-1947413 [Mycobacterium tuberculosis]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Nucleotide

• Length: 228 bp

>AT195418 NZ_CP094715:1923662-1923919 [Staphylococcus pasteuri]
ATGAAATTATATTTTCTACACGTAATTAATATTTTATTAACGATATTGTTTGTAATTTTTAATATCGTCATTACAAATAA
CACCAACCTAGATGACACCTTATGGTTAGTGCCCGGATTAATAGTATGTGGTTTGATTATTATTATAAGCTTATTTATAG
CTATTTCTAATAAGGATTTATTAAGTGAAATTCTCTTTTTTATTAATATCATTCTTACCCTATATTACATTTATCCAATT
TTTTACGATTTTCTTTAA

Structures

Antitoxin

• PDB: 7E4J
• AlphaFold DB: A0A7U4FAN9