Detailed information of TA system TA195411

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1239412..1239980
• Replicon: chromosome
• Accession: NZ_CP071128
• Organism: Mycobacterium tuberculosis strain 267/47W148

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: JZL63_RS05840
• Protein ID: WP_003405865.1
• Coordinates: 1239606..1239980 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: JZL63_RS05835
• Protein ID: WP_003405863.1
• Coordinates: 1239412..1239609 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JZL63_RS05815	1235453..1236091	-	639	WP_003898730.1	lipid droplet-associated protein	-
JZL63_RS05820	1236181..1237188	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
JZL63_RS05825	1237205..1238188	-	984	WP_003898731.1	hypothetical protein	-
JZL63_RS05830	1238251..1239324	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
JZL63_RS05835	1239412..1239609	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JZL63_RS05840	1239606..1239980	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
JZL63_RS05845	1240183..1240881	+	699	WP_003898733.1	hypothetical protein	-
JZL63_RS05850	1240999..1241184	+	186	WP_003901093.1	hypothetical protein	-
JZL63_RS05855	1241111..1241386	-	276	WP_003405867.1	hypothetical protein	-
JZL63_RS05860	1241629..1241952	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
JZL63_RS05865	1241967..1242677	-	711	WP_003911399.1	hypothetical protein	-
JZL63_RS05870	1242699..1242908	+	210	WP_003911400.1	hypothetical protein	-
JZL63_RS05875	1242860..1243567	-	708	Protein_1157	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T195411 WP_003405865.1 NZ_CP071128:1239606-1239980 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T195411 NZ_CP094714:850011-850364 [Staphylococcus schleiferi]
ATGAAGCGCGGGGACGTTTACTTAGCAGATTTATCACCAGTACAGGGCTCTGAACAGGGGGGAGTAAGGCCCGTAGTTAT
TATTCAGAATGACACTGGAAATAAATACAGTCCAACTGTTATTGTCGCAGCAATTACTGGTCGGATTAATAAAGCTAAAA
TACCAACGCATGTAGAAATAGAAAAAAATAAATATAAGCTCGACAAAGATTCTGTCATCTTATTAGAACAAATTCGAACG
GTCGATAAGAAGAGACTTAAAGAGAAATTAACATTTTTATCTGAAGAAAAGATGAAAGAAGTTAATTACGCACTTGGTAT
TAGTTTAGGACTGAACATGAATCATCATAAATAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT195411 WP_003405863.1 NZ_CP071128:1239412-1239609 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT195411 NZ_CP094714:849840-850010 [Staphylococcus schleiferi]
ATGTCAGCATTCAATCAAACAAGATTTAAAAATTTAGAACAATCTTTAAAAGAAGGCTATGTTCAAATGGCAGATTTGAA
TCTCTCCCTAGCAACTGAAGCATATTCAGTTGAATGTGAAGCCTGTGATTGTAATGAATCACACTTGTTATCAGATTATA
AGGATGACTAA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07