Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 41042..41778 Replicon plasmid p3347684I_1
Accession NZ_CP071081
Organism Klebsiella pneumoniae strain 3347684I

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag JY393_RS25680 Protein ID WP_003026803.1
Coordinates 41296..41778 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag JY393_RS25675 Protein ID WP_003026799.1
Coordinates 41042..41308 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JY393_RS25665 (JY393_25660) 37024..37602 - 579 WP_211652405.1 recombinase family protein -
JY393_RS25670 (JY393_25665) 37761..40745 + 2985 Protein_41 Tn3 family transposase -
JY393_RS25675 (JY393_25670) 41042..41308 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
JY393_RS25680 (JY393_25675) 41296..41778 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
JY393_RS25685 (JY393_25680) 41967..43256 + 1290 WP_257967146.1 ISNCY family transposase -
JY393_RS25690 (JY393_25685) 43416..44120 + 705 WP_211652406.1 toll/interleukin-1 receptor domain-containing protein -
JY393_RS25695 (JY393_25690) 44455..44712 - 258 WP_023292103.1 hypothetical protein -
JY393_RS27875 45354..45869 + 516 Protein_47 CSS-motif domain-containing protein -
JY393_RS27880 45933..46769 + 837 WP_228296467.1 EAL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..155851 155851
- inside IScluster/Tn - - 31143..43256 12113


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T195255 WP_003026803.1 NZ_CP071081:41296-41778 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T195255 NZ_CP094460:c335608-335243 [Burkholderia pyrrocinia]
ATGGTAAAAGCTCTCTTCGACACCAACATCCTGATCGACTATCTGGGTGGCGTGGAATCCGCCCGCGCGGAGCTAGGCCG
CTATGACTATCGTGCGATCAGCACGATCTCGTGGATGGAGGTTCTGGTCGGCACGTCCGCACGTAACGAAGCGCCGACTC
GCGCATGGCTCTCGTCATTCGATGTCATCGCGCTCGACAGCACGGTGGCGAATCGTGCCGTGATCATTCGCAAGGAACGC
CGCATACGCCTGCCCGACGCGATCGTCTGGGCATCGGCGCAAGTGAACGGGCTGCTGCTCGTGTCGCGCAACACGAAAGA
CTTCCCTCCCACCGAACCCGGTGTTCGAATGCCGTATCAGATCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT195255 WP_003026799.1 NZ_CP071081:41042-41308 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT195255 NZ_CP094460:c335820-335602 [Burkholderia pyrrocinia]
ATGAGCCGAATTCTGGTTGATCTGTCGGATGGCCAGCTCGACGAGTTGGCCGTCATCGTCGAGACTGAACGTCGTCCCCG
CGCCGCGATCATCCGCGACGCGATTGATGCCTACATTGCCCTGCACAAGCGCCCGCTCGCCGACGACGTATTCGGCCTCT
GGAAAGATCGCACGGTCGACGGGCTCGCGTATCAGGAGGAAATGCGCTCGGAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References