Detailed information of TA system TA195236

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 23773..24042
• Replicon: plasmid p3347558_4
• Accession: NZ_CP071077
• Organism: Escherichia coli strain 3347558

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: JY392_RS27385
• Protein ID: WP_001372321.1
• Coordinates: 23917..24042 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	23773..23838 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JY392_RS27355	19483..20010	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
JY392_RS27360	20068..20301	+	234	WP_000006003.1	DUF905 family protein	-
JY392_RS27365	20362..22385	+	2024	Protein_28	ParB/RepB/Spo0J family partition protein	-
JY392_RS27370	22454..22888	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
JY392_RS27375	22885..23647	+	763	Protein_30	plasmid SOS inhibition protein A	-
-	23616..23840	+	225	NuclAT_0	-	-
-	23616..23840	+	225	NuclAT_0	-	-
-	23616..23840	+	225	NuclAT_0	-	-
-	23616..23840	+	225	NuclAT_0	-	-
JY392_RS27380	23625..23804	-	180	WP_001309233.1	hypothetical protein	-
-	23773..23838	-	66	-	-	Antitoxin
JY392_RS28045	23826..23975	+	150	Protein_32	plasmid maintenance protein Mok	-
JY392_RS27385	23917..24042	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
JY392_RS27390	24361..24657	-	297	Protein_34	hypothetical protein	-
JY392_RS27395	24957..25253	+	297	WP_001272251.1	hypothetical protein	-
JY392_RS27400	25364..26185	+	822	WP_001234445.1	DUF932 domain-containing protein	-
JY392_RS27405	26482..27084	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
JY392_RS27410	27407..27790	+	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
JY392_RS27415	27984..28655	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
JY392_RS27420	28792..29019	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T195236 WP_001372321.1 NZ_CP071077:23917-24042 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T195236 NZ_CP094446:5845608-5845958 [Paenibacillus thiaminolyticus]
TTGATTGTAAAGCGCGGCGACGTTTTTTTTGCGGATTTATCGCCCGTCGTAGGTTCCGAGCAGGGAGGGGTGCGTCCCGT
TCTCGTCATTCAGAATGATATCGGGAACCGGTTCAGCCCAACCGTCATCGTGGCGGCAATTACGGCGCAGATTCAGAAGG
CCAAATTGCCGACCCATGTTGAAATTGATGCGAAGACGCATGGCTTCGACCGCGATTCCGTCATTTTGCTGGAACAGATC
CGCACCATCGACAAACAACGGCTCACTGACAAAATAACGCACTTAGATGAAGAGACGATGCGCAAGGTCGATGACGGGCT
GCTTATCAGCCTCGGATTGATTGATTTTTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT195236 NZ_CP071077:c23838-23773 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin