Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4254971..4255698 Replicon chromosome
Accession NZ_CP071073
Organism Escherichia coli strain 3347558

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag JY392_RS20630 Protein ID WP_000550189.1
Coordinates 4255384..4255698 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID D3GWQ0
Locus tag JY392_RS20625 Protein ID WP_000560253.1
Coordinates 4254971..4255387 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JY392_RS20615 (4250386) 4250386..4252737 + 2352 WP_000695439.1 alpha-glucosidase -
JY392_RS20620 (4252908) 4252908..4254926 + 2019 WP_000121438.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
JY392_RS20625 (4254971) 4254971..4255387 - 417 WP_000560253.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
JY392_RS20630 (4255384) 4255384..4255698 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
JY392_RS20635 (4255983) 4255983..4257119 - 1137 WP_000018677.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
JY392_RS20640 (4257204) 4257204..4257707 + 504 WP_001309773.1 M48 family metallopeptidase -
JY392_RS20645 (4257784) 4257784..4258467 + 684 WP_001183054.1 vancomycin high temperature exclusion protein -
JY392_RS20650 (4258546) 4258546..4259532 + 987 WP_001377074.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T195227 WP_000550189.1 NZ_CP071073:c4255698-4255384 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T195227 NZ_CP094443:c1221495-1221244 [Staphylococcus aureus]
ATGAATAACCGCGAACAAATTGAACAATCAATTATCAGTGCTAGTGCCTATAACGGTAATGACACAGAGGGATTACTAAA
AGAGGTTGAAGACGTGTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGATGGAATGACAAATGCTATTCAACATTCAG
TTAAAGAAGGTGTTGAACTTGATGAAGCAGTAGGGATTATGGCAGGTCAAGTTGTCTATAAATATGAGGAGGAACAGGAA
AATGAGTATTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14965.39 Da        Isoelectric Point: 4.4547

>AT195227 WP_000560253.1 NZ_CP071073:c4255387-4254971 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT195227 NZ_CP094443:c1221254-1221081 [Staphylococcus aureus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTATAAACTGTCCGATGATTCAGTTATTAGCATTATAGATTTTATTACTAAACCAATTTATCTAATTA
AGGGGGACGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure
AlphaFold DB A0A829IXD3

References