Detailed information of TA system TA195016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4622464..4623066
• Replicon: chromosome
• Accession: NZ_CP071013
• Organism: Enterobacter hormaechei strain RIVM_C033144

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155DUZ6
• Locus tag: JQL76_RS22005
• Protein ID: WP_022649837.1
• Coordinates: 4622755..4623066 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JQL76_RS22000
• Protein ID: WP_022649836.1
• Coordinates: 4622464..4622754 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQL76_RS21985	4619962..4620864	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
JQL76_RS21990	4620861..4621496	+	636	WP_006808711.1	formate dehydrogenase cytochrome b556 subunit	-
JQL76_RS21995	4621493..4622422	+	930	WP_022649835.1	formate dehydrogenase accessory protein FdhE	-
JQL76_RS22000	4622464..4622754	-	291	WP_022649836.1	helix-turn-helix domain-containing protein	Antitoxin
JQL76_RS22005	4622755..4623066	-	312	WP_022649837.1	hypothetical protein	Toxin
JQL76_RS22010	4623215..4624156	-	942	WP_022649838.1	fatty acid biosynthesis protein FabY	-
JQL76_RS22015	4624201..4624638	-	438	WP_022649839.1	D-tyrosyl-tRNA(Tyr) deacylase	-
JQL76_RS22020	4624635..4625516	-	882	WP_022649840.1	virulence factor BrkB family protein	-
JQL76_RS22025	4625510..4626109	-	600	WP_022649841.1	glucose-1-phosphatase	-
JQL76_RS22030	4626228..4627028	-	801	WP_022649842.1	DeoR/GlpR family DNA-binding transcription regulator	-
JQL76_RS22035	4627063..4627959	-	897	WP_022649843.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12148.13 Da
• Isoelectric Point: 9.4455

>T195016 WP_022649837.1 NZ_CP071013:c4623066-4622755 [Enterobacter hormaechei]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPDYGDLIQNTGGLRKIRWLAGGKGKRSGVRVIYFHRTRECEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T195016 NZ_CP094276:67847-68317 [Klebsiella aerogenes]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10699.21 Da
• Isoelectric Point: 10.5888

>AT195016 WP_022649836.1 NZ_CP071013:c4622754-4622464 [Enterobacter hormaechei]
MDKALFERLTQSIAQINEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALTQ

Nucleotide

• Length: 291 bp

>AT195016 NZ_CP094276:67407-67850 [Klebsiella aerogenes]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A155DUZ6