Detailed information of TA system TA194692

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 57888..58152
• Replicon: plasmid p40EC-2
• Accession: NZ_CP070922
• Organism: Escherichia coli strain 40EC

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: JR909_RS24105
• Protein ID: WP_001331364.1
• Coordinates: 58000..58152 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	57888..57945 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JR909_RS24090	53127..55418	-	2292	WP_001289276.1	hypothetical protein	-
JR909_RS24095	55411..56481	-	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
JR909_RS24100	56500..57708	-	1209	WP_000121274.1	IncI1-type conjugal transfer protein TrbA	-
-	57888..57945	-	58	NuclAT_0	-	Antitoxin
-	57888..57945	-	58	NuclAT_0	-	Antitoxin
-	57888..57945	-	58	NuclAT_0	-	Antitoxin
-	57888..57945	-	58	NuclAT_0	-	Antitoxin
JR909_RS24105	58000..58152	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
JR909_RS24110	58775..59071	+	297	WP_001275298.1	hypothetical protein	-
JR909_RS24115	59136..59312	-	177	WP_001054904.1	hypothetical protein	-
JR909_RS24120	59704..59913	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
JR909_RS24125	59985..60647	-	663	WP_205899293.1	plasmid IncI1-type surface exclusion protein ExcA	-
JR909_RS24130	60712..62874	-	2163	WP_000698351.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55	-	1..104226	104226

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T194692 WP_001331364.1 NZ_CP070922:58000-58152 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T194692 NZ_CP093930:1577682-1578143 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAGTAAGAGAGGCTGATGTACTTCCAGATAATTTAGA
CGGGGTATGGTTAGGAGATTTAATTTTAATAAAACGTGGTTTATCAGATGGAGAAAAAGCAGGAATTCTCTTCGAAGAAT
TAGCACATAATAAACTTACATACGGTGATATAGCCGATTACTCGAATTTCAACAATCGCAAGTTCGAAAATTACGCACGT
AGACATGGTTTTACTTCAGCTGTACCGCTACATGAAATTGTAGAAGCTCACAACTACGGTGTTCGTAATTTGTACGAGTT
GTCAGAGTATCTGCAGTTGAGTGAATCATACATACTAGAAGCTATAGAACAATATAAAAAGATATATGGTATTGGAACTC
ACTATGGCGAGTATTCTATTACGTTTGAGCCGTTGAGAGTTTTTAAATATAAGGAAATATAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT194692 NZ_CP070922:c57945-57888 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin