Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 859170..859824 Replicon chromosome
Accession NZ_CP070920
Organism Escherichia coli strain 40EC

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag JR909_RS04160 Protein ID WP_000244772.1
Coordinates 859417..859824 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JR909_RS04155 Protein ID WP_000354046.1
Coordinates 859170..859436 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR909_RS04130 854339..855082 + 744 WP_000951961.1 SDR family oxidoreductase -
JR909_RS04135 855139..856572 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
JR909_RS04140 856617..856928 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
JR909_RS04145 857092..857751 + 660 WP_000250274.1 hemolysin III family protein -
JR909_RS04150 857947..858927 - 981 WP_000886095.1 tRNA-modifying protein YgfZ -
JR909_RS04155 859170..859436 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JR909_RS04160 859417..859824 + 408 WP_000244772.1 protein YgfX Toxin
JR909_RS04165 859864..860385 - 522 WP_001055874.1 flavodoxin FldB -
JR909_RS04170 860497..861393 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JR909_RS04175 861418..862128 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JR909_RS04180 862134..863867 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T194667 WP_000244772.1 NZ_CP070920:859417-859824 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T194667 NZ_CP093919:1907595-1907846 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT194667 WP_000354046.1 NZ_CP070920:859170-859436 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT194667 NZ_CP093919:1907843-1908097 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References