Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3469640..3470258 Replicon chromosome
Accession NZ_CP070902
Organism Escherichia coli strain 99COLEC

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JR895_RS16680 Protein ID WP_001291435.1
Coordinates 3470040..3470258 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JR895_RS16675 Protein ID WP_000344800.1
Coordinates 3469640..3470014 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR895_RS16665 3464729..3465922 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JR895_RS16670 3465945..3469094 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
JR895_RS16675 3469640..3470014 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JR895_RS16680 3470040..3470258 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
JR895_RS16685 3470430..3470981 + 552 WP_000102564.1 maltose O-acetyltransferase -
JR895_RS16690 3471097..3471567 + 471 WP_001583270.1 YlaC family protein -
JR895_RS16695 3471731..3473281 + 1551 WP_086586081.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JR895_RS16700 3473323..3473676 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JR895_RS16710 3474055..3474366 + 312 WP_000409911.1 MGMT family protein -
JR895_RS16715 3474397..3474969 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T194611 WP_001291435.1 NZ_CP070902:3470040-3470258 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T194611 NZ_CP093910:c1289466-1289200 [Aggregatibacter actinomycetemcomitans]
ATGGATTACGTTTTATCAAAAGAATATAAACGGGACTTAAAAAAACTTCCGGTCGAAATTCAATCCGGTCCGGAATATGC
AGAGGTTTTATATTGCCTGTTCAATCAAAAATCCCTACCCGAACGATATAAAGATCATGCTTTGCAAGGAAATTGGCAGG
GATTTCGAGATTGCCACATCAAAAATGATCTCATTTTAATTTACAAAATAGAAGCAGACACCCTCTATTTTGCAAGGCTA
AATTCTCACTCGGAAGTGTTTAAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT194611 WP_000344800.1 NZ_CP070902:3469640-3470014 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT194611 NZ_CP093910:c1289750-1289478 [Aggregatibacter actinomycetemcomitans]
ATGTTAGATAGCGCAGTGAATTTCCGTACTCAAGCCGACATCAAAGAGCAAGCGTTTAACGTCATTAAAAGCTATGGCTT
AACGCCCGCTCAGGTGTTAAATATGTTTTTGACCCAAATTGCCAAAACCAACACAATTCCGCTCAGTTTGGATTATCAGC
CGAATACAAAAACAGCTAATGCAATCAATGAATTAATGAGCGGAAAAGGGGAAAGATTTAGCGTAGATTCCTTTGATGAA
TTTCAACAAAAAATGCGTGATTTAAGCAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References