Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1799298..1799800 Replicon chromosome
Accession NZ_CP070902
Organism Escherichia coli strain 99COLEC

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag JR895_RS08520 Protein ID WP_000767829.1
Coordinates 1799546..1799800 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag JR895_RS08515 Protein ID WP_001259255.1
Coordinates 1799298..1799549 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR895_RS08490 1794476..1795543 - 1068 WP_000080095.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
JR895_RS08495 1795543..1796613 - 1071 WP_053292574.1 histidinol-phosphate transaminase -
JR895_RS08500 1796610..1797914 - 1305 WP_000009594.1 histidinol dehydrogenase -
JR895_RS08505 1797920..1798819 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
JR895_RS08510 1798965..1799015 - 51 WP_001364200.1 his operon leader peptide -
JR895_RS08515 1799298..1799549 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
JR895_RS08520 1799546..1799800 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
JR895_RS08525 1799883..1800707 + 825 WP_000754737.1 SDR family oxidoreductase -
JR895_RS08530 1800753..1801682 + 930 WP_053292573.1 LysR family transcriptional regulator -
JR895_RS08535 1801897..1801959 + 63 WP_010723108.1 membrane protein YoeI -
JR895_RS08540 1801949..1803307 + 1359 WP_024250698.1 putrescine/proton symporter PlaP -
JR895_RS08545 1803486..1804544 + 1059 WP_000492311.1 transport protein YeeE -
JR895_RS08550 1804558..1804785 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T194600 WP_000767829.1 NZ_CP070902:1799546-1799800 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T194600 NZ_CP093856:c5376092-5375787 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTATATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCACCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT194600 WP_001259255.1 NZ_CP070902:1799298-1799549 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT194600 NZ_CP093856:c5376312-5376094 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References