Detailed information of TA system TA194572

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 199575..200314
• Replicon: chromosome
• Accession: NZ_CP070898
• Organism: Klebsiella pneumoniae strain JNQH116

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: JYA99_RS00975
• Protein ID: WP_205733658.1
• Coordinates: 199829..200314 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: JYA99_RS00970
• Protein ID: WP_003026799.1
• Coordinates: 199575..199841 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JYA99_RS00955	195078..197147	+	2070	WP_002922127.1	glycine--tRNA ligase subunit beta	-
JYA99_RS00960	197444..198813	+	1370	WP_087830479.1	IS3 family transposase	-
JYA99_RS00965	199014..199442	+	429	WP_004901287.1	GFA family protein	-
JYA99_RS00970	199575..199841	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
JYA99_RS00975	199829..200314	+	486	WP_205733658.1	GNAT family N-acetyltransferase	Toxin
JYA99_RS00980	200658..200810	+	153	WP_002922102.1	type I toxin-antitoxin system toxin HokA	-
JYA99_RS00985	201112..202731	+	1620	WP_032415799.1	ATP-binding cassette domain-containing protein	-
JYA99_RS00990	202830..203042	-	213	WP_000014594.1	RNA chaperone/antiterminator CspA	-
JYA99_RS00995	203295..203585	-	291	WP_002921931.1	HTH-type transcriptional regulator	-
JYA99_RS01000	203831..205186	-	1356	WP_032415797.1	aromatic acid/H+ symport family MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	198088..198813	725

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17638.42 Da
• Isoelectric Point: 10.3370

>T194572 WP_205733658.1 NZ_CP070898:199829-200314 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALSAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T194572 NZ_CP093854:c231906-231688 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT194572 WP_003026799.1 NZ_CP070898:199575-199841 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT194572 NZ_CP093854:c232307-231933 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66