Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3425832..3426394 Replicon chromosome
Accession NZ_CP070873
Organism Escherichia fergusonii strain EF91

Toxin (Protein)


Gene name higB Uniprot ID E9XKD5
Locus tag JW972_RS16325 Protein ID WP_000605673.1
Coordinates 3426116..3426394 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag JW972_RS16320 Protein ID WP_000781370.1
Coordinates 3425832..3426116 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW972_RS16305 (3421191) 3421191..3424238 + 3048 WP_015912497.1 formate dehydrogenase-N subunit alpha -
JW972_RS16310 (3424251) 3424251..3425135 + 885 WP_001240580.1 formate dehydrogenase N subunit beta -
JW972_RS16315 (3425128) 3425128..3425781 + 654 WP_000045645.1 formate dehydrogenase-N subunit gamma -
JW972_RS16320 (3425832) 3425832..3426116 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
JW972_RS16325 (3426116) 3426116..3426394 - 279 WP_000605673.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JW972_RS16330 (3426580) 3426580..3427590 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
JW972_RS16335 (3427725) 3427725..3429422 - 1698 WP_000433464.1 malate dehydrogenase -
JW972_RS16340 (3429578) 3429578..3429715 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
JW972_RS16345 (3429817) 3429817..3430032 - 216 WP_000495766.1 biofilm-dependent modulation protein -
JW972_RS16350 (3430375) 3430375..3430806 + 432 WP_000152299.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10535.13 Da        Isoelectric Point: 7.3562

>T194544 WP_000605673.1 NZ_CP070873:c3426394-3426116 [Escherichia fergusonii]
MIMNFRHKGLRDLFLLGKTSGVIPTQIKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T194544 NZ_CP093548:4388181-4388579 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT194544 WP_000781370.1 NZ_CP070873:c3426116-3425832 [Escherichia fergusonii]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT194544 NZ_CP093548:4387885-4388178 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7ADF6

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References