Detailed information of TA system TA194352

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 719..1193
• Replicon: plasmid p51248_2
• Accession: NZ_CP070566
• Organism: Klebsiella pneumoniae strain KP51248

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: JW300_RS00630
• Protein ID: WP_004197762.1
• Coordinates: 719..985 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: JW300_RS00635
• Protein ID: WP_000879771.1
• Coordinates: 969..1193 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW300_RS00625	299..649	+	351	WP_004197756.1	hypothetical protein	-
JW300_RS00630	719..985	-	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JW300_RS00635	969..1193	-	225	WP_000879771.1	TraY domain-containing protein	Antitoxin
JW300_RS00640	1860..2072	-	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
JW300_RS00645	2079..2588	-	510	WP_032433972.1	MbeB family mobilization protein	-
JW300_RS00650	2563..3327	-	765	Protein_6	relaxase/mobilization nuclease domain-containing protein	-
JW300_RS00655	3266..3610	-	345	WP_004197764.1	MobC family plasmid mobilization relaxosome protein	-
JW300_RS00660	3600..3776	-	177	WP_165764108.1	hypothetical protein	-
JW300_RS00665	4879..5260	+	382	Protein_9	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5598	5598

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T194352 WP_004197762.1 NZ_CP070566:c985-719 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T194352 NZ_CP093445:3558666-3558884 [Salmonella enterica subsp. enterica serovar Abeokuta]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT194352 WP_000879771.1 NZ_CP070566:c1193-969 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT194352 NZ_CP093445:3558264-3558638 [Salmonella enterica subsp. enterica serovar Abeokuta]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Antitoxin

• AlphaFold DB: S1EJF5