Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3400162..3400810 Replicon chromosome
Accession NZ_CP070549
Organism Citrobacter freundii strain CF49969

Toxin (Protein)


Gene name higB Uniprot ID A0A0D7M266
Locus tag JW296_RS18945 Protein ID WP_016151211.1
Coordinates 3400448..3400810 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0D7LEH2
Locus tag JW296_RS18940 Protein ID WP_003840660.1
Coordinates 3400162..3400461 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW296_RS18920 3396484..3396900 - 417 WP_003023951.1 GNAT family N-acetyltransferase -
JW296_RS18925 3397031..3397324 + 294 WP_003840665.1 hypothetical protein -
JW296_RS18930 3397419..3398801 + 1383 WP_016151210.1 glycoside hydrolase family 1 protein -
JW296_RS18935 3398850..3400043 - 1194 WP_003840662.1 purine ribonucleoside efflux pump NepI -
JW296_RS18940 3400162..3400461 - 300 WP_003840660.1 helix-turn-helix transcriptional regulator Antitoxin
JW296_RS18945 3400448..3400810 - 363 WP_016151211.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JW296_RS18950 3400911..3401363 - 453 WP_003023933.1 DUF1198 family protein -
JW296_RS18955 3401489..3402880 - 1392 WP_003023929.1 hexose-6-phosphate:phosphate antiporter -
JW296_RS18960 3403023..3404351 - 1329 WP_003023927.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13788.66 Da        Isoelectric Point: 5.1976

>T194321 WP_016151211.1 NZ_CP070549:c3400810-3400448 [Citrobacter freundii]
MWEVETTDAFDKWFDVQTEALKEDMLAAMMILSEYGPQLGRPFADTVNASAFSNMKELRVQHQGSPIRAFFAFDPSRHGI
VLCAGDKTGLNEKKFYKEMIRLADAEYRNHLISKENYGYP

Download         Length: 363 bp

>T194321 NZ_CP093407:4684624-4684935 [Salmonella enterica subsp. enterica serovar Agona]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTAACTGCATATTATCGGGGTAATAAAGAATGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11016.85 Da        Isoelectric Point: 8.5047

>AT194321 WP_003840660.1 NZ_CP070549:c3400461-3400162 [Citrobacter freundii]
MATLKELMAKQSPESQERITEKVEILRQAVALNMLREELNLSQAELASAMGVKQPTIAKMEQADNDPRLSTLKRYVTALG
GELSIDVKLPTGKRVAFYL

Download         Length: 300 bp

>AT194321 NZ_CP093407:4684932-4685378 [Salmonella enterica subsp. enterica serovar Agona]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGCTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTCAAACTACCAGCAGATGCCTTCATCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D7M266

Antitoxin

Source ID Structure
AlphaFold DB A0A0D7LEH2

References