Detailed information of TA system TA194266

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1846294..1846884
• Replicon: chromosome
• Accession: NZ_CP070528
• Organism: Klebsiella pneumoniae strain KP47693

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: JW292_RS09695
• Protein ID: WP_023341911.1
• Coordinates: 1846552..1846884 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: JW292_RS09690
• Protein ID: WP_000288812.1
• Coordinates: 1846294..1846551 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW292_RS09670	1842629..1844377	+	1749	WP_032418698.1	hypothetical protein	-
JW292_RS09675	1844431..1845012	+	582	WP_099184326.1	hypothetical protein	-
JW292_RS09680	1845295..1845756	+	462	WP_004213450.1	hypothetical protein	-
JW292_RS09685	1845753..1845959	+	207	WP_020805021.1	helix-turn-helix domain-containing protein	-
JW292_RS09690	1846294..1846551	+	258	WP_000288812.1	antitoxin	Antitoxin
JW292_RS09695	1846552..1846884	+	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JW292_RS09705	1847206..1848642	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
JW292_RS09715	1849008..1850462	-	1455	WP_004148975.1	AMP nucleosidase	-
JW292_RS09720	1850592..1850837	-	246	WP_004141189.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T194266 WP_023341911.1 NZ_CP070528:1846552-1846884 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T194266 NZ_CP093401:28886-29368 [Salmonella enterica subsp. enterica serovar Infantis]
ATGGAAATAAGTGTTACTGCACCGGAGTTGCTAAACGAGGAACACTATCTTCAACAGTTTGATTGCGGGAATGATGTGCT
CAGTGACTGGCTTCGCCGCAGAGCGATGAAGAACCAATACCTCAATGCCTCCCGTACATTTGTTATCTGCCCTGAAGGCA
CAAAGCGAGTAGTGGGATATTACTCTATTGCGACGGGGTCTGTAAGTCATGCCAGTTTAGGTCGCAGTTTACGCCAGAAT
ATGCCCGATCCTGTTCCGGTTGTTTTGCTGGGACGTCTTGCTGTTGATGAGTGTACTCAAGGGCATAGTTTTGGAAAATG
GCTGCTTAATGATGCTGTAACACGGGTATCTAACCTGGCAGACCAGGTGGGTATTAAAGCGATCATGGTTCATGCTATTG
ATGAACAGGCGAAAACATTCTATGAATACTTTGGCTTTGTTCAGTCTCCCATTGCGCCAAATACATTGTTCTACAAGATT
TAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT194266 WP_000288812.1 NZ_CP070528:1846294-1846551 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT194266 NZ_CP093401:28611-28895 [Salmonella enterica subsp. enterica serovar Infantis]
ATGCAAACTACAATCCGCAAATCTGTGCGTAATAAACAAATAAATATACGGGCTACTGATGAGGAACGTGCTGTGATTGA
TTATGCAGCGAGCCTGGTCAGTAAAAACCGTACCGACTTCATCATAGAAAAGGCTGTGAGCGAGGCCCAGAATATCATTC
TGGATCAACGCGTTTTTGTTCTGGATGATGCCCGCTATCAGGCTTTTATTAAACAACTTGAAGCTCCTGTACAGAATACT
GAAGGTCGTCAGCGCCTGATGGATGTGAAACCTGAATGGAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0