Detailed information of TA system TA194064

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2678450..2680038
• Replicon: chromosome
• Accession: NZ_CP070449
• Organism: Klebsiella pneumoniae strain B18064

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: JT745_RS13100
• Protein ID: WP_064162061.1
• Coordinates: 2678450..2679772 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: JT745_RS13105
• Protein ID: WP_002904790.1
• Coordinates: 2679772..2680038 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JT745_RS13085	2673506..2676007	+	2502	WP_064162059.1	fimbrial biogenesis outer membrane usher protein	-
JT745_RS13090	2675995..2676999	+	1005	WP_064162060.1	type 1 fimbrial protein	-
JT745_RS13095	2677141..2678433	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
JT745_RS13100	2678450..2679772	-	1323	WP_064162061.1	type II toxin-antitoxin system HipA family toxin	Toxin
JT745_RS13105	2679772..2680038	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JT745_RS13110	2680304..2680630	-	327	WP_064162062.1	hydroxyisourate hydrolase	-
JT745_RS13115	2680627..2681127	-	501	WP_064162063.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
JT745_RS13120	2681105..2682484	-	1380	WP_040245186.1	purine permease	-
JT745_RS13125	2682830..2683984	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
JT745_RS13130	2683965..2684888	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49155.46 Da
• Isoelectric Point: 7.5357

>T194064 WP_064162061.1 NZ_CP070449:c2679772-2678450 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSTHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T194064 NZ_CP093335:c3746690-3745443 [Sphingopyxis granuli]
ATGAGCGACGCGCTCGCCGTCTATTGGGACGACCGGCGTGTCGGTACGCTCGCGCGCAACGCCGAGGGCACGATGTTGTT
TTGCTACGATGACCTGTGGCTCGCCGATCCCGCCTTCAGGCCGATTTCGGCCTCGCTCCCCCGCCAGACCGGGCCATTCA
GCCGCGCCGCGACCCGCCCTTTCTTCGCCGGTCTCCTTCCCGATGAAGAACAGCGCGCCGCCGCGGCGCGCGCCGCCGGT
GTCTCGGCCCAGAATGATTATGCGCTGCTCGACGCCCTCGGCGGCGACGTCGCCGGCGCATTGGTCATCGTGCCGGAAGG
GCAGGCGGTACCCGCAGCTTCGGGCACGGCCCCCGACCCGCTGACCGAGGCGGCGCTCGCGGCGCTCCTCGAGGAACTGC
CGCGCCGTCCCTTTCTCGTTGGCGACGAGGGCATACGCATGTCGCTCGCTGGCGCGCAGCCCAAGCTGCCGGTCGTTCTC
GTCGCGGGCGCTCCCACGCTGCCGTCTCCGGGGCAGCCTACCACCCACATCGTCAAGCCCGCCATGTCCCGCTTCGCGCA
CAGCGTCGAAACGGAAGCCTTCGCGATGCGACTGGCCGCCGCCGCCGGTCTCGCCGTGGCGGCGGTCGAGCCGCGCCGCG
CTTCGGCCATCCCCTATCTTCTCGTCGAGCGATACGACCGCAGCGACGCTGGGGGCACGATCCGGCGTATCCACCAGGAG
GATTTCTGCCAGGCGCTCGGCATCGCGCCCGAACATAAATATGCCATCGAGGGCGGGCCCGGCTTTGCCGCCTGCTTCGA
TCTCGTTCGCCGCACCTGCACTGCTCCGGCCCGCGACATCCTGCGCCTGCTCGATGCGGCCATATTCAACGCAGTGGTCG
GCAACGCCGATGCGCATGGCAAGAATTTCAGCCTCCTGTACCGCGACGATGCGACCGTCCTCGCTCCGCTCTACGATCTC
ATGGTCACGGCCCATTATCCCGAGGTCGCCACCCGCTTCGCGATGCCGATCGGCCGCGCCAACCAGCTCGACGGTCTCGA
TGCGCGCGCATGGGAACGCCTTGCCACCGACACAGGGCTCGGCGCCCCCTTCGTCCGCCGCCGTGTCCAGGCCCTCGCTG
ACGGCATCCGCGAGGCCGCACCGGGCATCGCCGACGCGCTCGCTCACGAAGGCTGCGATCCCGATGTTCTCGCGTCGCTC
GTGGCGCTCGTCGGACACCGGGCCGATGCCATAGCGGCCGCGCTCTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT194064 WP_002904790.1 NZ_CP070449:c2680038-2679772 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT194064 NZ_CP093335:c3746905-3746687 [Sphingopyxis granuli]
ATGGATATCACGACCCTGGGGCAATTGGTGCGCCGTGTGCGCAAACAGGCTGGTCTTCGCCAGGATGAACTCGCTGGCGC
GGCGGGCGTGGGTATACGTTTCATCGTCGATCTCGAAAGCGGCAAGTCCACGCTGCAGATCGGCAAGGTGCTGCATGTCC
TCGATACGCTGGGTATCGCCGCTACCTTCTCGGCGCCCGGAGAGGACAGCGTCGGATGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GXD8