Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5277879..5278504 Replicon chromosome
Accession NZ_CP070415
Organism Klebsiella pneumoniae strain B1347

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag JT725_RS25705 Protein ID WP_002882817.1
Coordinates 5277879..5278262 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JT725_RS25710 Protein ID WP_004150355.1
Coordinates 5278262..5278504 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JT725_RS25690 5275245..5276147 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JT725_RS25695 5276144..5276779 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JT725_RS25700 5276776..5277705 + 930 WP_064162495.1 formate dehydrogenase accessory protein FdhE -
JT725_RS25705 5277879..5278262 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
JT725_RS25710 5278262..5278504 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
JT725_RS25715 5278709..5279626 + 918 WP_004181612.1 alpha/beta hydrolase -
JT725_RS25720 5279640..5280581 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JT725_RS25725 5280626..5281063 - 438 WP_064162494.1 D-tyrosyl-tRNA(Tyr) deacylase -
JT725_RS25730 5281060..5281920 - 861 WP_004146232.1 virulence factor BrkB family protein -
JT725_RS25735 5281914..5282513 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T193951 WP_002882817.1 NZ_CP070415:c5278262-5277879 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T193951 NZ_CP093298:c1871260-1870910 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT193951 WP_004150355.1 NZ_CP070415:c5278504-5278262 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT193951 NZ_CP093298:c1871547-1871266 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References