Detailed information of TA system TA193669

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 130306..130729
• Replicon: plasmid pESA134_2
• Accession: NZ_CP070286
• Organism: Escherichia albertii strain Sample 169

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: JRC46_RS24725
• Protein ID: WP_223200913.1
• Coordinates: 130306..130428 (-)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	130513..130729 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JRC46_RS24695 (125876)	125876..126351	-	476	Protein_136	IS3 family transposase	-
JRC46_RS24700 (126515)	126515..127081	-	567	WP_204678076.1	hypothetical protein	-
JRC46_RS25385 (127217)	127217..127519	-	303	WP_244259633.1	hypothetical protein	-
JRC46_RS25390 (127516)	127516..127728	-	213	WP_244259634.1	hypothetical protein	-
JRC46_RS25395 (128078)	128078..128431	-	354	WP_244259645.1	hypothetical protein	-
JRC46_RS24715 (128905)	128905..129897	+	993	Protein_141	IS481 family transposase	-
JRC46_RS24725 (130306)	130306..130428	-	123	WP_223200913.1	Hok/Gef family protein	Toxin
JRC46_RS25400 (130373)	130373..130525	-	153	Protein_143	DUF5431 family protein	-
- (130513)	130513..130729	-	217	NuclAT_0	-	Antitoxin
- (130513)	130513..130729	-	217	NuclAT_0	-	Antitoxin
- (130513)	130513..130729	-	217	NuclAT_0	-	Antitoxin
- (130513)	130513..130729	-	217	NuclAT_0	-	Antitoxin
JRC46_RS24730 (130698)	130698..131460	-	763	Protein_144	plasmid SOS inhibition protein A	-
JRC46_RS24735 (131457)	131457..131891	-	435	WP_000845916.1	conjugation system SOS inhibitor PsiB	-
JRC46_RS24740 (131946)	131946..133895	-	1950	Protein_146	ParB/RepB/Spo0J family partition protein	-
JRC46_RS24745 (133961)	133961..134194	-	234	WP_000005990.1	DUF905 family protein	-
JRC46_RS24750 (134252)	134252..134731	-	480	WP_204620444.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	espC / espC	1..176494	176494

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4538.38 Da
• Isoelectric Point: 8.2691

>T193669 WP_223200913.1 NZ_CP070286:c130428-130306 [Escherichia albertii]
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 123 bp

>T193669 NZ_CP093070:c5483-5208 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACTGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Nucleotide

• Length: 217 bp

>AT193669 NZ_CP070286:c130729-130513 [Escherichia albertii]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTCTTTTCAGGAAAAGTGAGTGTGGTCAGCGCGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGC
AGAAAGCCCCGTAGTTAATTTTCATTAACCCACGAGGCCTCAGCATGTCTAGTCCAC

Structures

Antitoxin