Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 652892..653619 Replicon chromosome
Accession NZ_CP070232
Organism Escherichia coli strain 10R

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag JTF63_RS03130 Protein ID WP_000550189.1
Coordinates 652892..653206 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JTF63_RS03135 Protein ID WP_000560266.1
Coordinates 653203..653619 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTF63_RS03110 649050..650036 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
JTF63_RS03115 650115..650807 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
JTF63_RS03120 650884..651387 - 504 WP_001333820.1 M48 family metallopeptidase -
JTF63_RS03125 651472..652608 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
JTF63_RS03130 652892..653206 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
JTF63_RS03135 653203..653619 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
JTF63_RS03140 653664..655682 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
JTF63_RS03145 656108..658459 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T193575 WP_000550189.1 NZ_CP070232:652892-653206 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T193575 NZ_CP093031:c922278-922096 [Pseudomonas aeruginosa]
ATGAAGTTCAGCGAATTCAGACGATGGTTGAAGGCCCAAGGGGTGACCTTCGAAGCCGGCAAGGGAAGCCACTTCAAGAT
CACCGCCCCGAACGGCAAACAGACCACCTTCGCGGACCACGGAGCTAAGGAAATGCCAGAACCGACCCGCAAGGCGATCA
TCAAGCAACTGGGGCTCAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT193575 WP_000560266.1 NZ_CP070232:653203-653619 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT193575 NZ_CP093031:c922049-921621 [Pseudomonas aeruginosa]
ATGTACGACTATGCAATCCGTTTCGAACAGGACGATAGCGCTCCTGGCGTCGCCGTTTTCTGCAGAGACCTGCCGGAACT
GAACAGCTACGGCGACGATAAGGCCCATGCAATCAGCGAGGCGGTTGACGCCATCGAGTCAACCCTCTCGCTGTACGTTG
ATCAGCGCCGAGAAATCCCCGCAGCCAGCCAGGCACAACCAGGCGAGCGCGTTATCCATCTGCCGGCGGTTACCGTTGCG
AAGGTCGCGCTCTGGAACGAAATGATCCGTCGAGATATGCGAAAAGCTGACCTCTGCCGGCTCCTCGGGATCGCGCAGAT
CCAGGGTGACAGGCTCGTCGACTTCCTCCACAACACCAAGATGGAAGCCATGGAGAACGCGCTATCCGCCCTCGGCCTCC
GCCTATCAGTGAATATCGAGGCGGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References