Detailed information of TA system TA193463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1099933..1100660
• Replicon: chromosome
• Accession: NZ_CP070170
• Organism: Escherichia coli strain FDAARGOS_1284

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: I6K14_RS05175
• Protein ID: WP_000547563.1
• Coordinates: 1099933..1100244 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K14_RS05180
• Protein ID: WP_000126304.1
• Coordinates: 1100241..1100660 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K14_RS05145 (1095086)	1095086..1096795	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
I6K14_RS05150 (1096805)	1096805..1097347	+	543	WP_000493799.1	formate hydrogenlyase subunit HycF	-
I6K14_RS05155 (1097347)	1097347..1098114	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
I6K14_RS05160 (1098111)	1098111..1098521	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
I6K14_RS05165 (1098514)	1098514..1098981	+	468	WP_021522200.1	hydrogenase maturation peptidase HycI	-
I6K14_RS05170 (1099015)	1099015..1099770	+	756	WP_001314074.1	hypothetical protein	-
I6K14_RS05175 (1099933)	1099933..1100244	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
I6K14_RS05180 (1100241)	1100241..1100660	+	420	WP_000126304.1	helix-turn-helix domain-containing protein	Antitoxin
I6K14_RS05185 (1100752)	1100752..1101180	-	429	WP_000536064.1	hypothetical protein	-
I6K14_RS05190 (1101565)	1101565..1102092	+	528	WP_001078777.1	electron transport protein HydN	-
I6K14_RS05195 (1102245)	1102245..1104497	+	2253	WP_085455499.1	carbamoyltransferase HypF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T193463 WP_000547563.1 NZ_CP070170:1099933-1100244 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T193463 NZ_CP092986:c35019-34678 [Leptospira interrogans]
TTGGTAAAGAATAGAAACTATACTCCTGAAAAAGGAGATATAGTGTGGCTGAACTTTACTCCTCAAGCAGGTCATGAACA
GAAAGGTCGTAGACCTGCACTTGTTTTATCGCCTAAGGAATATAATTCAAAGACAGGGTTAGCAATATTTTGTCCAATTA
CCAGTAAAATTAAAGGTTATCCATTCGAAGTGTTGATTAAATCAAAAAAAATCGATGGTGTAATTCTTTCTGATCAAGTA
AAAAACTTAGATTGGACTATCCGTGAAGCAGAATTTATTGAATCAATAAATAAAGTTTCTTTAAAAGAAGTTTTAGATAA
TATTAAATTATTAATATTCTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15474.50 Da
• Isoelectric Point: 4.4596

>AT193463 WP_000126304.1 NZ_CP070170:1100241-1100660 [Escherichia coli]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT193463 NZ_CP092986:c35249-35013 [Leptospira interrogans]
ATGGAATCAATTATTCAAAAATGGGGTAATAGCTTAGGAATTAGAATTCCAAAAGCAATGGCAACGGAATTAGAGTTAAA
TGATGGAAGCCATGTTGAATTACAATATGAAGGAGATAAGATTGTAATATATCCAATGAAAAAAGCTTCATTGGAAGATA
AACTTTCTAAAATTACGAAACAAAATCTTCACTCTGAAATATCTACTGGTAATTCTATAGGCAATGAAGCTTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22