Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 2647627..2648222 Replicon chromosome
Accession NZ_CP070157
Organism Escherichia coli strain FDAARGOS_1276

Toxin (Protein)


Gene name chpB Uniprot ID A0A0V9NWK6
Locus tag I6K06_RS12695 Protein ID WP_019842229.1
Coordinates 2647627..2647977 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID L4JJX7
Locus tag I6K06_RS12700 Protein ID WP_001223213.1
Coordinates 2647971..2648222 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K06_RS12675 (2642957) 2642957..2643979 - 1023 WP_001550507.1 ABC transporter permease -
I6K06_RS12680 (2643993) 2643993..2645495 - 1503 WP_001550506.1 sugar ABC transporter ATP-binding protein -
I6K06_RS12685 (2645627) 2645627..2646583 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
I6K06_RS12690 (2646893) 2646893..2647423 + 531 WP_000055075.1 inorganic diphosphatase -
I6K06_RS12695 (2647627) 2647627..2647977 - 351 WP_019842229.1 endoribonuclease toxin ChpB Toxin
I6K06_RS12700 (2647971) 2647971..2648222 - 252 WP_001223213.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
I6K06_RS12705 (2648434) 2648434..2648775 - 342 WP_001550504.1 gamma-glutamylcyclotransferase -
I6K06_RS12710 (2648778) 2648778..2652557 - 3780 WP_001550503.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-115)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12483.44 Da        Isoelectric Point: 5.6097

>T193384 WP_019842229.1 NZ_CP070157:c2647977-2647627 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVWMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Download         Length: 351 bp

>T193384 NZ_CP092907:2340668-2341012 [Enterococcus faecalis]
ATGAAAAGTATCAAAGAGTTGGTAGAAGAATATAATGTGGAGTTAGTTTTTACTACTTTGAACAAACGCGCATGTTTCGA
CCCTACCTACGGTATCATATTTGTAAATCAAAATTTAACACCATCAGAACAAGAAGAAGCAATATATCACGAGTTAAAGC
ATGTAAAAGACCATGTGGATATAATGGCATTGTATAAAGTTCCTGTTTTTCGTTCTAAGATGGAATCCGAAGCAGAACAA
TATATGTTTAGAAGCTTAATCGAAAAATATGAAGGACAATACAATTACTCAAATGTTATAGCTCATTACAACTTAAAAAT
GGGACAAGAAGTTTATTTGAAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9331.64 Da        Isoelectric Point: 4.6509

>AT193384 WP_001223213.1 NZ_CP070157:c2648222-2647971 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT193384 NZ_CP092907:2340326-2340649 [Enterococcus faecalis]
ATGACTGTATTTGATAGAGTAAAAAAATTAGCAGATAGTCAGAAAATATCTATTGTCGAACTTGAAGAAAAGTTAAATTT
CAGTCGAAATTCATTATACGCTTGGAAAAAAAGTAAACCTTCTATTGATAAATTAGAAGCTGTTGCAAATTATTTTGGAG
TTTCAACAGATTATTTATTAGGTCGTGAAGTTTCTAATAAATCAAAGCAATCTGATGATTTAGATGATGTACTGGATAAC
GTCATGAGTTTTGACGGTGAACCACTTGATGATCATGACAGAGAAGTTATCCGGGCATATTTAAAGGGTAGATTCGGGAA
ATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NWK6

Antitoxin

Source ID Structure
AlphaFold DB L4JJX7

References