Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3652856..3653687 Replicon chromosome
Accession NZ_CP070115
Organism Escherichia coli strain FDAARGOS_1298

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag I6K28_RS17605 Protein ID WP_000854814.1
Coordinates 3652856..3653230 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag I6K28_RS17610 Protein ID WP_001285586.1
Coordinates 3653319..3653687 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K28_RS17565 (3648252) 3648252..3649418 + 1167 WP_001297905.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
I6K28_RS17570 (3649537) 3649537..3650010 + 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
I6K28_RS17575 (3650208) 3650208..3651266 + 1059 WP_001200890.1 FUSC family protein -
I6K28_RS17580 (3651438) 3651438..3651767 + 330 WP_000450409.1 DUF496 family protein -
I6K28_RS17585 (3651868) 3651868..3652002 - 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
I6K28_RS17590 (3652104) 3652104..3652250 + 147 Protein_3463 transposase domain-containing protein -
I6K28_RS17595 (3652539) 3652539..3652619 - 81 Protein_3464 hypothetical protein -
I6K28_RS17600 (3652665) 3652665..3652859 - 195 WP_000988601.1 DUF5983 family protein -
I6K28_RS17605 (3652856) 3652856..3653230 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
I6K28_RS17610 (3653319) 3653319..3653687 - 369 WP_001285586.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
I6K28_RS17615 (3653761) 3653761..3653982 - 222 WP_000692323.1 DUF987 domain-containing protein -
I6K28_RS17620 (3654051) 3654051..3654527 - 477 WP_001351157.1 RadC family protein -
I6K28_RS17625 (3654543) 3654543..3655028 - 486 WP_000213703.1 antirestriction protein -
I6K28_RS17630 (3655119) 3655119..3655940 - 822 WP_001234569.1 DUF932 domain-containing protein -
I6K28_RS17635 (3656161) 3656161..3656571 - 411 WP_000846704.1 hypothetical protein -
I6K28_RS17640 (3656587) 3656587..3657264 - 678 WP_001362823.1 hypothetical protein -
I6K28_RS17645 (3657400) 3657400..3658470 - 1071 WP_000102631.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T193211 WP_000854814.1 NZ_CP070115:c3653230-3652856 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T193211 NZ_CP092780:2879826-2880008 [Massilia sp. MB5]
ATGAACTCCAAAACCTTAATTGATATGCTGAAGGCAGATGGATGGCAGCTTGTACGGATCAGAGGCAGCCATCATCATTT
CAGGCATCCTGGAAAGAATGGTTTGATTACCGTGCCGCATCCCAAGAAGGATTTACCAGCAGGGACGTGGCGTCGCATCT
TGCATGATGCTGGGTTGAGGTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT193211 WP_001285586.1 NZ_CP070115:c3653687-3653319 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT193211 NZ_CP092780:2880020-2880445 [Massilia sp. MB5]
ATGGATCTTCCGGTCATAATTTTTAAGGATGAAGGCAGCGTATATGGCGTGAGCGTGCCGGATATCAGCGGCTGCCATTC
ATGGGGAGACACGATTGGTGACGCGCTTCGCAACGTGAAAGAGGCCATTGCCAGTCACATCGAAACGCTGCTGGAGCTTG
GCGAAAACATTGAGGTGACGCCGTCGGATATTGAGTGTTTGCGTGAGCGTCCGGAATACGAAGGCGCTATCTGGGCGCTT
GTGAATGTCGATCTGAGCAAGCTTGATAGCAAACCTGAGAGGATCAATATCAGCTTGCCCCGCTTTGTCCTCAGCAAGAT
CGATAAGTACGCAGATGAACGTCATGAAACCCGCAGCGGTTTGCTGGCGCGGGCTGCCGTAAAACTGATTAACGACGAAA
CGGCCCTGAAAACAAGCAAAACGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References