Detailed information of TA system TA192985

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1643039..1643874
• Replicon: chromosome
• Accession: NZ_CP070077
• Organism: Escherichia coli strain FDAARGOS_1371

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0A1A9A5
• Locus tag: I6L01_RS07815
• Protein ID: WP_001564063.1
• Coordinates: 1643497..1643874 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0A1AEL8
• Locus tag: I6L01_RS07810
• Protein ID: WP_038432125.1
• Coordinates: 1643039..1643407 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L01_RS07775 (1638698)	1638698..1639378	+	681	WP_001278649.1	WYL domain-containing protein	-
I6L01_RS07780 (1639529)	1639529..1640206	+	678	WP_001564058.1	hypothetical protein	-
I6L01_RS07785 (1640212)	1640212..1640445	+	234	WP_001278293.1	DUF905 family protein	-
I6L01_RS07790 (1640535)	1640535..1641353	+	819	WP_001175165.1	DUF932 domain-containing protein	-
I6L01_RS07795 (1641619)	1641619..1642098	+	480	WP_001564060.1	antirestriction protein	-
I6L01_RS07800 (1642114)	1642114..1642590	+	477	WP_021553055.1	RadC family protein	-
I6L01_RS07805 (1642655)	1642655..1642876	+	222	WP_000692345.1	DUF987 domain-containing protein	-
I6L01_RS07810 (1643039)	1643039..1643407	+	369	WP_038432125.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6L01_RS07815 (1643497)	1643497..1643874	+	378	WP_001564063.1	TA system toxin CbtA family protein	Toxin
I6L01_RS07820 (1643871)	1643871..1644020	+	150	Protein_1537	DUF5983 family protein	-
I6L01_RS07825 (1644099)	1644099..1644293	+	195	WP_024181941.1	DUF957 domain-containing protein	-
I6L01_RS07830 (1644378)	1644378..1645220	+	843	Protein_1539	DUF4942 domain-containing protein	-
I6L01_RS07835 (1645969)	1645969..1647507	+	1539	WP_204628255.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1609477..1655319	45842

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14094.09 Da
• Isoelectric Point: 7.8045

>T192985 WP_001564063.1 NZ_CP070077:1643497-1643874 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRSNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T192985 NZ_CP092639:981491-981826 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13569.31 Da
• Isoelectric Point: 7.0369

>AT192985 WP_038432125.1 NZ_CP070077:1643039-1643407 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGNRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT192985 NZ_CP092639:981243-981491 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0A1A9A5

Antitoxin

• AlphaFold DB: A0A0A1AEL8