Detailed information of TA system TA192768

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 24509..24762
• Replicon: plasmid unnamed
• Accession: NZ_CP070044
• Organism: Escherichia coli strain FDAARGOS_1303

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: I6K33_RS00145
• Protein ID: WP_001336447.1
• Coordinates: 24509..24658 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	24706..24762 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K33_RS00105 (19957)	19957..20727	+	771	WP_000712166.1	molybdopterin-dependent oxidoreductase	-
I6K33_RS00110 (20757)	20757..21032	+	276	WP_048220931.1	pentapeptide MXKDX repeat protein	-
I6K33_RS00115 (21182)	21182..21280	-	99	WP_137491101.1	transposase	-
I6K33_RS00120 (21473)	21473..21781	+	309	Protein_23	transposase	-
I6K33_RS00130 (22809)	22809..23666	-	858	WP_204672452.1	incFII family plasmid replication initiator RepA	-
I6K33_RS00135 (23659)	23659..23733	-	75	WP_001365705.1	RepA leader peptide Tap	-
I6K33_RS00140 (23967)	23967..24224	-	258	WP_000084404.1	replication regulatory protein RepA	-
I6K33_RS00145 (24509)	24509..24658	-	150	WP_001336447.1	Hok/Gef family protein	Toxin
- (24706)	24706..24762	+	57	NuclAT_1	-	Antitoxin
- (24706)	24706..24762	+	57	NuclAT_1	-	Antitoxin
- (24706)	24706..24762	+	57	NuclAT_1	-	Antitoxin
- (24706)	24706..24762	+	57	NuclAT_1	-	Antitoxin
I6K33_RS00150 (24857)	24857..25294	-	438	WP_000872609.1	hypothetical protein	-
I6K33_RS00155 (25447)	25447..25830	-	384	WP_001109264.1	hypothetical protein	-
I6K33_RS00160 (25933)	25933..26394	-	462	WP_000760080.1	thermonuclease family protein	-
I6K33_RS00165 (27147)	27147..27350	-	204	WP_001336517.1	hypothetical protein	-
I6K33_RS00170 (27502)	27502..28059	-	558	WP_000139329.1	fertility inhibition protein FinO	-
I6K33_RS00175 (28114)	28114..28860	-	747	WP_000205709.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..86762	86762
	-	flank	IS/Tn	-	-	21515..21781	266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T192768 WP_001336447.1 NZ_CP070044:c24658-24509 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T192768 NZ_CP092538:c2499148-2498882 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAGGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT192768 NZ_CP070044:24706-24762 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin