Detailed information of TA system TA192644

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 53343..53582
• Replicon: plasmid unnamed1
• Accession: NZ_CP070008
• Organism: Escherichia coli strain FDAARGOS_1288

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: I6K18_RS25900
• Protein ID: WP_023144756.1
• Coordinates: 53343..53477 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	53522..53582 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K18_RS25855 (48929)	48929..49069	-	141	Protein_46	DUF262 domain-containing protein	-
I6K18_RS25860 (49354)	49354..49755	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
I6K18_RS25865 (49688)	49688..49945	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
I6K18_RS25870 (50038)	50038..50691	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
I6K18_RS25875 (50789)	50789..50929	-	141	WP_001333237.1	hypothetical protein	-
I6K18_RS25880 (51631)	51631..52488	-	858	WP_000130640.1	incFII family plasmid replication initiator RepA	-
I6K18_RS25885 (52481)	52481..52555	-	75	WP_031943482.1	RepA leader peptide Tap	-
I6K18_RS25895 (52792)	52792..53046	-	255	WP_000083850.1	replication regulatory protein RepA	-
I6K18_RS25900 (53343)	53343..53477	-	135	WP_023144756.1	Hok/Gef family protein	Toxin
- (53522)	53522..53582	+	61	NuclAT_1	-	Antitoxin
- (53522)	53522..53582	+	61	NuclAT_1	-	Antitoxin
- (53522)	53522..53582	+	61	NuclAT_1	-	Antitoxin
- (53522)	53522..53582	+	61	NuclAT_1	-	Antitoxin
I6K18_RS25905 (53549)	53549..53835	-	287	Protein_55	DUF2726 domain-containing protein	-
I6K18_RS25910 (54348)	54348..54560	-	213	WP_013023861.1	hypothetical protein	-
I6K18_RS25915 (54691)	54691..55251	-	561	WP_000139341.1	fertility inhibition protein FinO	-
I6K18_RS25920 (55306)	55306..56052	-	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / dfrA14 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2	-	1..198318	198318
	-	flank	IS/Tn	-	-	48176..48553	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T192644 WP_023144756.1 NZ_CP070008:c53477-53343 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T192644 NZ_CP092496:67058-67474 [Citrobacter freundii]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTACGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCTGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCCAGTGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGATGCTGG
AAGACTGGGTAAAATAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT192644 NZ_CP070008:53522-53582 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin