Detailed information of TA system TA192639

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4424275..4425863
• Replicon: chromosome
• Accession: NZ_CP070007
• Organism: Escherichia coli strain FDAARGOS_1288

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: U9YVT8
• Locus tag: I6K18_RS21345
• Protein ID: WP_001125415.1
• Coordinates: 4424541..4425863 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: I6K18_RS21340
• Protein ID: WP_001296726.1
• Coordinates: 4424275..4424541 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K18_RS21340 (4424275)	4424275..4424541	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
I6K18_RS21345 (4424541)	4424541..4425863	+	1323	WP_001125415.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
I6K18_RS21350 (4426261)	4426261..4427160	-	900	WP_001057776.1	LysR family transcriptional regulator	-
I6K18_RS21355 (4427300)	4427300..4427608	+	309	WP_001221046.1	hypothetical protein	-
I6K18_RS21360 (4427617)	4427617..4428306	+	690	WP_000801313.1	type 1 glutamine amidotransferase domain-containing protein	-
I6K18_RS21365 (4428380)	4428380..4429327	+	948	WP_000854796.1	aldo/keto reductase	-
I6K18_RS21370 (4429430)	4429430..4429585	+	156	WP_001322415.1	hypothetical protein	-
I6K18_RS21375 (4429545)	4429545..4429964	+	420	WP_001322416.1	amidohydrolase family protein	-
I6K18_RS21380 (4429954)	4429954..4430106	+	153	WP_001322417.1	amidohydrolase family protein	-
I6K18_RS21385 (4430257)	4430257..4430829	+	573	WP_000789913.1	NAD(P)H-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49384.05 Da
• Isoelectric Point: 8.5830

>T192639 WP_001125415.1 NZ_CP070007:4424541-4425863 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREYGIK

Nucleotide

• Length: 1323 bp

>T192639 NZ_CP092493:4593881-4594258 [Citrobacter freundii]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT192639 WP_001296726.1 NZ_CP070007:4424275-4424541 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT192639 NZ_CP092493:4593465-4593791 [Citrobacter freundii]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCTTCGGCTCCTGTGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAA
AAAGTAA

Structures

Toxin

• AlphaFold DB: U9YVT8