Detailed information of TA system TA192458

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 60808..61061
• Replicon: plasmid unnamed
• Accession: NZ_CP069979
• Organism: Escherichia coli strain FDAARGOS_1299

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: I6K29_RS24775
• Protein ID: WP_001336447.1
• Coordinates: 60912..61061 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	60808..60864 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K29_RS24750 (56710)	56710..57456	+	747	WP_000205709.1	conjugal transfer pilus acetylase TraX	-
I6K29_RS24755 (57511)	57511..58068	+	558	WP_000139329.1	fertility inhibition protein FinO	-
I6K29_RS25370 (58220)	58220..58423	+	204	WP_001336517.1	hypothetical protein	-
I6K29_RS24760 (59176)	59176..59637	+	462	WP_000760080.1	thermonuclease family protein	-
I6K29_RS24765 (59740)	59740..60123	+	384	WP_001109264.1	hypothetical protein	-
I6K29_RS24770 (60276)	60276..60713	+	438	WP_000872609.1	hypothetical protein	-
- (60808)	60808..60864	-	57	NuclAT_1	-	Antitoxin
- (60808)	60808..60864	-	57	NuclAT_1	-	Antitoxin
- (60808)	60808..60864	-	57	NuclAT_1	-	Antitoxin
- (60808)	60808..60864	-	57	NuclAT_1	-	Antitoxin
I6K29_RS24775 (60912)	60912..61061	+	150	WP_001336447.1	Hok/Gef family protein	Toxin
I6K29_RS24780 (61346)	61346..61603	+	258	WP_000084404.1	replication regulatory protein RepA	-
I6K29_RS24785 (61837)	61837..61911	+	75	WP_001365705.1	RepA leader peptide Tap	-
I6K29_RS24790 (61904)	61904..62761	+	858	WP_021534656.1	incFII family plasmid replication initiator RepA	-
I6K29_RS24800 (63789)	63789..64097	-	309	Protein_83	transposase	-
I6K29_RS24805 (64290)	64290..64388	+	99	WP_137491101.1	transposase	-
I6K29_RS24810 (64538)	64538..64804	-	267	WP_204638966.1	hypothetical protein	-
I6K29_RS24815 (64834)	64834..65604	-	771	WP_000712166.1	molybdopterin-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..99190	99190
	-	flank	IS/Tn	-	-	63789..64055	266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T192458 WP_001336447.1 NZ_CP069979:60912-61061 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T192458 NZ_CP092410:c166084-165614 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACAGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Nucleotide

• Length: 57 bp

>AT192458 NZ_CP069979:c60864-60808 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin